pH-, temperature- and ion-dependent oligomerization of Sulfolobus solfataricus recombinant amidase: a study with site-specific mutants [0.03%]
酸碱度、温度及离子依赖的硫化叶菌重组氨酶寡聚化：位点特异性突变体研究
Laura Politi,Emilia Chiancone,Laura Giangiacomo et al.
Laura Politi et al.
Recombinant amidase from Sulfolobus solfataricus occurred as a dimer of 110 kDa comprising identical subunits. Only dimers were present at pHs above 7.0, but with decreasing pH, dimers associated into octamers, with complete oligomerization...
Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase [0.03%]
改善Sulfolobus acidocaldarius 2-酮-3-脱氧葡萄糖酸醛缩酶的低温活性
Suzanne Wolterink-van Loo,Marco A J Siemerink,Georgios Perrakis et al.
Suzanne Wolterink-van Loo et al.
Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase (SacKdgA) displays optimal activity at 95 degrees C and is studied as a model enzyme for aldol condensation reactions. For application of SacKdgA at lower temperatures, a library of...
Many nonuniversal archaeal ribosomal proteins are found in conserved gene clusters [0.03%]
许多非通用古菌核糖体蛋白位于保守基因簇中
Jiachen Wang,Indrani Dasgupta,George E Fox
Jiachen Wang
The genomic associations of the archaeal ribosomal proteins, (r-proteins), were examined in detail. The archaeal versions of the universal r-protein genes are typically in clusters similar or identical and to those found in bacteria. Of the...