A reliable method for quantification of splice variants using RT-qPCR [0.03%]
一种使用RT-qPCR量化剪接变异的可靠方法
Julia Camacho Londoño,Stephan E Philipp
Julia Camacho Londoño
Background: The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine t...
Gene expression profiling of the venom gland from the Venezuelan mapanare (Bothrops colombiensis) using expressed sequence tags (ESTs) [0.03%]
使用表达序列标签（EST）对委内瑞拉马帕纳雷毒腺基因表达谱进行测序分析
Montamas Suntravat,Néstor L Uzcategui,Chairat Atphaisit et al.
Montamas Suntravat et al.
Background: Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70% of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, t...
Knockdown of SALL4 inhibits the proliferation and reverses the resistance of MCF-7/ADR cells to doxorubicin hydrochloride [0.03%]
SALL4基因沉默抑制MCF-7/ADR细胞增殖及逆转其盐酸多柔比星耐药性作用的研究
Yuan-Yuan Chen,Zhi-Zhen Li,Yuan-Yuan Ye et al.
Yuan-Yuan Chen et al.
Background: Breast cancer is the most frequent malignancy in women and drug resistance is the major obstacle for its successful chemotherapy. In the present study, we analyzed the involvement of an oncofetal gene, sal-lik...
What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme [0.03%]
什么是正常？下一代测序驱动的人类循环miRNA组分析研究
D P Tonge,T W Gant
D P Tonge
Background: MicroRNAs (miRNAs) are short non-protein-coding RNA species that have a regulatory function in modulating protein translation and degradation of specific mRNAs. MicroRNAs are estimated to target approximately ...
TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint [0.03%]
TLK1B介导的Rad9磷酸化调节其核/细胞质定位和细胞周期检查点功能
Sanket Awate,Arrigo De Benedetti
Sanket Awate
Background: The Tousled like kinase 1B (TLK1B) is critical for DNA repair and survival of cells. Upon DNA damage, Chk1 phosphorylates TLK1B at S457 leading to its transient inhibition. Once TLK1B regains its kinase activi...
Erratum to: Temperature-induced variation in gene expression burst size in metazoan cells [0.03%]
correction to: 后螠虫动物细胞中温度引起的基因表达爆发大小的差异
Ophélie Arnaud,Sam Meyer,Elodie Vallin et al.
Ophélie Arnaud et al.
Published Erratum
BMC molecular biology. 2016 Feb 3:17:2. DOI:10.1186/s12867-015-0054-4 2016
Structural and functional analysis of four non-coding Y RNAs from Chinese hamster cells: identification, molecular dynamics simulations and DNA replication initiation assays [0.03%]
中国仓鼠细胞四种无编码Y RNA的结构和功能分析：鉴定、分子动力学模拟及DNA复制起始试验
Quirino Alves de Lima Neto,Francisco Ferreira Duarte Junior,Paulo Sérgio Alves Bueno et al.
Quirino Alves de Lima Neto et al.
Background: The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no informat...
microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4 [0.03%]
miR-150通过靶向FOXO4促进宫颈癌细胞的生长和存活
Jun Li,Lina Hu,Chao Tian et al.
Jun Li et al.
Background: Dysregulation of microRNA-150 (miR-150) is commonly observed in solid tumor and has been reported to be involved in multiple important biological processes, such as cell proliferation, apoptosis, and metastasi...
Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation [0.03%]
雄激素受体转染曲细精管支持细胞可改变基因表达而无需雄激素刺激
D Fietz,M Markmann,D Lang et al.
D Fietz et al.
Background: Androgens play an important role for the development of male fertility and gained interest as growth and survival factors for certain types of cancer. Androgens act via the androgen receptor (AR/Ar), which is ...
Ana Raquel Soares,Noémia Fernandes,Marisa Reverendo et al.
Ana Raquel Soares et al.
Background: Small non-coding RNAs (sncRNAs) are a class of transcripts implicated in several eukaryotic regulatory mechanisms, namely gene silencing and chromatin regulation. Despite significant progress in their identifi...