An optimized rapid bisulfite conversion method with high recovery of cell-free DNA [0.03%]
一种高效回收游离DNA的快速优化转化方法
Shaohua Yi,Fei Long,Juanbo Cheng et al.
Shaohua Yi et al.
Background: Methylation analysis of cell-free DNA is a encouraging tool for tumor diagnosis, monitoring and prognosis. Sensitivity of methylation analysis is a very important matter due to the tiny amounts of cell-free DN...
Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939 [0.03%]
RanBP2介导的p27kiplSUMO化促进癌细胞在肝内胆管癌QBC939细胞系中的增殖
Jun Yang,Yan Liu,Bing Wang et al.
Jun Yang et al.
Background: Cholangiocarcinoma is one of the deadly disease with poor 5-year survival and poor response to conventional therapies. Previously, we found that p27kip1 nuclear-cytoplasmic translocation confers proliferation ...
An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing [0.03%]
一种优化的从石蜡包埋组织激光捕获显微切割小片段中提取适用于下一代测序RNA的方法
Parisa Amini,Julia Ettlin,Lennart Opitz et al.
Parisa Amini et al.
Background: Formalin-fixed paraffin embedded (FFPE) tissue constitutes a vast treasury of samples for biomedical research. Thus far however, extraction of RNA from FFPE tissue has proved challenging due to chemical RNA-pr...
Physical shearing imparts biological activity to DNA and ability to transmit itself horizontally across species and kingdom boundaries [0.03%]
物理剪切赋予DNA生物活性并使其能够在物种和界之间水平传播
Gorantla Venkata Raghuram,Deepika Gupta,Siddharth Subramaniam et al.
Gorantla Venkata Raghuram et al.
Background: We have recently reported that cell-free DNA (cfDNA) fragments derived from dying cells that circulate in blood are biologically active molecules and can readily enter into healthy cells to activate DNA damage...
Interaction between NFATc2 and the transcription factor Sp1 in pancreatic carcinoma cells PaTu 8988t [0.03%]
NFATc2与转录因子Sp1在人胰腺癌细胞系PaTu 8988t中的相互作用研究
Manuela Malsy,Bernhard Graf,Katrin Almstedt
Manuela Malsy
Background: Nuclear factors of activated T-cells (NFATs) have been mainly characterized in the context of immune response regulation because, as transcription factors, they have the ability to induce gene transcription. N...
Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA [0.03%]
通过拼接阵列发现新的RBM10靶点，包括SMN2前体mRNA
Leslie C Sutherland,Philippe Thibault,Mathieu Durand et al.
Leslie C Sutherland et al.
Background: RBM10 is an RNA binding protein involved in message stabilization and alternative splicing regulation. The objective of the research described herein was to identify novel targets of RBM10-regulated splicing. ...
Growth arrest specific gene 2 in tilapia (Oreochromis niloticus): molecular characterization and functional analysis under low-temperature stress [0.03%]
尼罗罗非鱼生长阻滞特异性基因2的分子特征及低温胁迫下的功能分析
ChangGeng Yang,Fan Wu,Xing Lu et al.
ChangGeng Yang et al.
Background: Growth arrest specific 2 (gas2) gene is a component of the microfilament system that plays a major role in the cell cycle, regulation of microfilaments, and cell morphology during apoptotic processes. However,...
Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands [0.03%]
鉴定具有转录调节功能的Dele和Cdc6 CpG岛G-四链体结构
Daniyah H Bay,Annika Busch,Fred Lisdat et al.
Daniyah H Bay et al.
Background: G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands D...
Mitochondrial RNA processing in absence of tRNA punctuations in octocorals [0.03%]
无tRNA标点符号的情况下八放珊瑚线粒体RNA的加工过程
Gaurav G Shimpi,Sergio Vargas,Angelo Poliseno et al.
Gaurav G Shimpi et al.
Background: Mitogenome diversity is staggering among early branching animals with respect to size, gene density, content and order, and number of tRNA genes, especially in cnidarians. This last point is of special interes...
Microarray expression profiling in the denervated hippocampus identifies long noncoding RNAs functionally involved in neurogenesis [0.03%]
微阵列表达谱分析鉴定出在去神经支配的海马体中功能上参与神经发生的长链非编码RNA
Bingying Deng,Xiang Cheng,Haoming Li et al.
Bingying Deng et al.
Background: The denervated hippocampus provides a proper microenvironment for the survival and neuronal differentiation of neural progenitors. While thousands of lncRNAs were identified, only a few lncRNAs that regulate n...