Coincidence cloning recovery of Brucella melitensis RNA from goat tissues: advancing the in vivo analysis of pathogen gene expression in brucellosis [0.03%]
小山羊组织中利用巧合克隆恢复梅里氏布鲁杆菌RNA：促进布病中病原体基因表达的体内分析
Paola M Boggiatto,Daniel Fitzsimmons,Darrell O Bayles et al.
Paola M Boggiatto et al.
Background: Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the...
Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression [0.03%]
正面共因子4（PC4）参与调控复制依赖的规范组蛋白基因表达
Aleksandra Brzek,Marlena Cichocka,Jakub Dolata et al.
Aleksandra Brzek et al.
Background: Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this...
Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions [0.03%]
评估在不同实验条件下适合作草莓（Fragaria × ananassa）qRT-PCR标准化的参考基因
Yunting Zhang,Xiaorui Peng,Yi Liu et al.
Yunting Zhang et al.
Background: Strawberry has received much attention due to its nutritional value, unique flavor, and attractive appearance. The availability of the whole genome sequence and multiple transcriptome databases allows the grea...
Laser capture microdissection for transcriptomic profiles in human skin biopsies [0.03%]
激光捕获切割技术在人皮肤活检样本转录组分析中的应用
Silvia Santoro,Ignazio Diego Lopez,Raffaella Lombardi et al.
Silvia Santoro et al.
Background: The acquisition of reliable tissue-specific RNA sequencing data from human skin biopsy represents a major advance in research. However, the complexity of the process of isolation of specific layers from fresh-...
Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides [0.03%]
使用锁核酸寡核苷酸在胃癌细胞中靶向抑制miR-9表达的研究
Joana Filipa Lima,Joana Carvalho,Inês Pinto-Ribeiro et al.
Joana Filipa Lima et al.
Background: Gastric cancer is the third leading cause of cancer-related mortality worldwide. Recently, it has been demonstrated that gastric cancer cells display a specific miRNA expression profile, with increasing eviden...
Quantitative profiling of BATF family proteins/JUNB/IRF hetero-trimers using Spec-seq [0.03%]
利用Spec-seq对BATF家族蛋白/JUNB/IRF异三聚体进行定量谱分析
Yiming K Chang,Zheng Zuo,Gary D Stormo
Yiming K Chang
Background: BATF family transcription factors (BATF, BATF2 and BATF3) form hetero-trimers with JUNB and either IRF4 or IRF8 to regulate cell fate in T cells and dendritic cells in vivo. While each combination of the heter...
pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor [0.03%]
SPI-B介导的酸激活AQP1基因表达调控机制研究
Yihui Zhai,Hong Xu,Qian Shen et al.
Yihui Zhai et al.
Background: Bicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underly...
A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone [0.03%]
一种使用pcDNA3载体骨架在哺乳动物细胞中通过CRISPR-Cas9系统截短目标基因的质粒构建方法
Neftali Vazquez,Lilia Sanchez,Rebecca Marks et al.
Neftali Vazquez et al.
Background: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employ...
Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples [0.03%]
石蜡包埋的人类肾活检样品的微离散肾小球突起的mRNA分析建议
Clemens L Bockmeyer,Juliane Wittig,Karen Säuberlich et al.
Clemens L Bockmeyer et al.
Background: Glomeruli are excellent pre-determined natural structures for laser micro-dissection. Compartment-specific glomerular gene expression analysis of formalin-fixed paraffin-embedded renal biopsies could improve r...
Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5'-phosphate-dependent exonuclease in Candida albicans and other yeasts [0.03%]
营养缺乏和TOR抑制在白色念珠菌和其他酵母中诱导产生一种抵抗5’-磷酸酶依赖性核酸外切酶的18S和25S核糖体RNA
Jacob Fleischmann,Miguel A Rocha
Jacob Fleischmann
Background: Messenger RNA (mRNA) represents a small percentage of RNAs in a cell, with ribosomal RNA (rRNA) making up the bulk of it. To isolate mRNA from eukaryotes, typically poly-A selection is carried out. Recently, a...