Differential roles of tryptophan residues in the functional expression of human anion exchanger 1 (AE1, Band 3, SLC4A1) [0.03%]
人阴离子交换器1(AE1、Band 3、SLC4A1)功能表达中色氨酸残基的不同作用
Yuka Okawa,Jing Li,Arghya Basu et al.
Yuka Okawa et al.
Anion exchanger 1 (AE1) is a 95 kDa glycoprotein that facilitates Cl(-)=HCO(-)(3) exchange across the erythrocyte plasma membrane. This transport activity resides in the 52 kDa C-terminal membrane domain (Gly(361)-Val(911)) predicted to spa...
Brij detergents reveal new aspects of membrane microdomain in erythrocytes [0.03%]
新型洗涤剂揭示红细胞膜微域的新特征
Bruna Renata Casadei,Patrícia De Oliveira Carvalho,Karin A Riske et al.
Bruna Renata Casadei et al.
Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resista...
Topology mapping to characterize cyanobacterial bicarbonate transporters: BicA (SulP/SLC26 family) and SbtA [0.03%]
用于表征蓝藻碳酸氢盐转运蛋白拓扑结构的图谱：BicA（SulP/SLC26家族）和SbtA
G Dean Price,Susan M Howitt
G Dean Price
This mini-review addresses advances in understanding the transmembrane topologies of two unrelated, single-subunit bicarbonate transporters from cyanobacteria, namely BicA and SbtA. BicA is a Na(+)-dependent bicarbonate transporter that bel...
Bacterial lipid modification of proteins requires appropriate secretory signals even for expression - implications for biogenesis and protein engineering [0.03%]
细菌脂质修饰蛋白质需要适当的分泌信号，即使在表达时也是如此-对生物发生和蛋白质工程的影响
Subramani Kumar,M M Balamurali,Krishnan Sankaran
Subramani Kumar
Sec- and Tat-mediated bacterial lipid modification of proteins are important posttranslational processes owing to their vital roles in cellular functions, membrane targeting and biotechnological applications like ELISA, biosensor, adjuvant-...
Generating thermostabilized agonist-bound GPR40/FFAR1 using virus-like particles and a label-free binding assay [0.03%]
基于病毒样颗粒的全人源GPR40受体结构模板及新型配基结合检测方法
Yoshihiko Hirozane,Takashi Motoyaji,Takamitsu Maru et al.
Yoshihiko Hirozane et al.
Elucidating the detailed mechanism of activation of membrane protein receptors and their ligand binding is essential for structure-based drug design. Membrane protein crystal structure analysis successfully aids in understanding these funda...
Microscopy approaches to investigate protein dynamics and lipid organization [0.03%]
用于研究蛋白质动态学和脂质组织的显微镜技术
Joanna M Kwiatek,Elizabeth Hinde,Katharina Gaus
Joanna M Kwiatek
The structure of cell membranes has been intensively investigated and many models and concepts have been proposed for the lateral organization of the plasma membrane. While proteomics and lipidomics have identified many if not all membrane ...
The blood-brain barrier: structure, function and therapeutic approaches to cross it [0.03%]
血脑屏障：结构、功能及穿越它的治疗策略
Marta Tajes,Eva Ramos-Fernández,Xian Weng-Jiang et al.
Marta Tajes et al.
The blood-brain barrier (BBB) is constituted by a specialized vascular endothelium that interacts directly with astrocytes, neurons and pericytes. It protects the brain from the molecules of the systemic circulation but it has to be overcom...
Modeling FRET to investigate the selectivity of lactose permease of Escherichia coli for lipids [0.03%]
建立FRET模型以研究乳糖透酶对脂质的选择性
Carme Suárez-Germà,Jordi Hernández-Borrell,Manuel Prieto et al.
Carme Suárez-Germà et al.
Förster resonance energy transfer (FRET) is a photophysical process by which a donor (D) molecule in an electronic excited state transfers its excitation energy to a second species, the acceptor (A). Since FRET efficiency depends on D-A se...
TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor [0.03%]
利用TROSY技术研究52千道尔顿的糖转运蛋白及其与小分子抑制剂的结合现象
Arnout P Kalverda,James Gowdy,Gary S Thompson et al.
Arnout P Kalverda et al.
Using the sugar transport protein, GalP, from Escherichia coli, which is a homologue of human GLUT transporters, we have overcome the challenges for achieving high-resolution [(15)N-(1)H]- and [(13)C-(1)H]-methyl-TROSY NMR spectra with a 52...
The Sec translocon mediated protein transport in prokaryotes and eukaryotes [0.03%]
细菌和真核生物中Sec转运蛋白介导的蛋白质运输
Kärt Denks,Andreas Vogt,Ilie Sachelaru et al.
Kärt Denks et al.
Protein transport via the Sec translocon represents an evolutionary conserved mechanism for delivering cytosolically-synthesized proteins to extra-cytosolic compartments. The Sec translocon has a three-subunit core, termed Sec61 in Eukaryot...