The Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope [0.03%]
鲨鱼的第二次袭击：鲨鱼IgNAR抗体可变区的超可变环2及其作为自主抗原结合位点的潜力
Stefan Zielonka,Martin Empting,Doreen Könning et al.
Stefan Zielonka et al.
In this present study, we engineered hypervariable loop 2 (HV2) of the IgNAR variable domain in a way that it solely facilitates antigen binding, potentially functioning as an autonomous paratope. For this, the surface-exposed loop correspo...
Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish [0.03%]
在斑马鱼中Gal4/UAS系统转录活性和DNA甲基化动态变化
Shao-Chen Pang,Hou-Peng Wang,Zuo-Yan Zhu et al.
Shao-Chen Pang et al.
The Gal4/upstream activating sequence (UAS) system is a powerful genetic tool for the temporal and spatial expression of target genes. In this study, the dynamic activity of the Gal4/UAS system was monitored in zebrafish throughout the enti...
Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin [0.03%]
来源于海洋的黑酵母Aureobasidium melanogenum P16的基因改良，高效生产聚葡萄糖的过程中利用菊粉的例子
Zai-Chao Ma,Nan-Nan Liu,Zhe Chi et al.
Zai-Chao Ma et al.
In this study, in order to directly and efficiently convert inulin into pullulan, the INU1 gene from Kluyveromyces maximum KM was integrated into the genomic DNA and actively expressed in the high pullulan producer Aureobasidium melanogenum...
Identification of Sex-Specific Markers Reveals Male Heterogametic Sex Determination in Pseudobagrus ussuriensis [0.03%]
性别特异性标记的鉴定揭示了乌苏里条鳅的雄性异配性别决定系统
Zheng-Jun Pan,Xi-Yin Li,Feng-Jian Zhou et al.
Zheng-Jun Pan et al.
Comprehending sex determination mechanism is a first step for developing sex control breeding biotechnologies in fish. Pseudobagrus ussuriensis, one of bagrid catfishes in Bagridae, had been observed to have about threefold size dimorphism ...
Identification of Sex-Linked SNPs and Sex-Determining Regions in the Yellowtail Genome [0.03%]
黄尾鱼基因组中性别连锁的SNP和性别决定区域的鉴定
Takashi Koyama,Akiyuki Ozaki,Kazunori Yoshida et al.
Takashi Koyama et al.
Unlike the conservation of sex-determining (SD) modes seen in most mammals and birds, teleost fishes exhibit a wide variety of SD systems and genes. Hence, the study of SD genes and sex chromosome turnover in fish is one of the most interes...
Microarray Analysis of Immunity Against WSSV in Response to Injection of Non-specific Long dsRNA in Kuruma Shrimp, Marsupenaeus japonicus [0.03%]
通过注射非特异性长双链RNA对 kuruma 虾 (Marsupenaeus japonicus) 抗 WSSV 免疫反应的芯片分析研究
Benedict Arias Maralit,Mami Komatsu,Sheryll Grospe Hipolito et al.
Benedict Arias Maralit et al.
Injection of shrimp with non-specific double-stranded RNA (dsRNA) of diverse lengths, sequences, and base compositions is known to induce non-specific immunity and protect against lethal disease, although the mechanisms are unclear. Previou...
Molecular Cloning, Characterization, and Expression Analysis of a Prolyl 4-Hydroxylase from the Marine Sponge Chondrosia reniformis [0.03%]
来源于海洋海绵Chondrosia reniformis的脯氨酰4-羟化酶的分子克隆、特征分析及表达研究
Marina Pozzolini,Sonia Scarfì,Francesca Mussino et al.
Marina Pozzolini et al.
Prolyl 4-hydroxylase (P4H) catalyzes the hydroxylation of proline residues in collagen. P4H has two functional subunits, α and β. Here, we report the cDNA cloning, characterization, and expression analysis of the α and β subunits of the...
Chondroitin Lyase from a Marine Arthrobacter sp. MAT3885 for the Production of Chondroitin Sulfate Disaccharides [0.03%]
海洋Arthrobacter_MAT3885菌株的软骨素酶及其用于生产硫酸软骨素双糖的应用研究
Varsha Kale,Ólafur Friðjónsson,Jón Óskar Jónsson et al.
Varsha Kale et al.
Chondroitin sulfate (CS) saccharides from cartilage tissues have potential application in medicine or as dietary supplements due to their therapeutic bioactivities. Studies have shown that depolymerized CS saccharides may display enhanced b...
A chymotrypsin from the Digestive Tract of California Spiny Lobster, Panulirus interruptus: Purification and Biochemical Characterization [0.03%]
加州带刺龙虾（Panulirus interruptus）消化道的胃蛋白酶：纯化及生化性质研究
Betsaida Bibo-Verdugo,Liliana Rojo-Arreola,Maria A Navarrete-del-Toro et al.
Betsaida Bibo-Verdugo et al.
A chymotrypsin was purified from the gastric juice of California spiny lobster (Panulirus interrutpus), using preparative electrophoresis and affinity chromatography on agarose-p-aminobenzamidine. The molecular mass was estimated by polyacr...
Quantitative Analysis of Carbon Flow into Photosynthetic Products Functioning as Carbon Storage in the Marine Coccolithophore, Emiliania huxleyi [0.03%]
定量分析海洋颗石藻（艾米利亚·赫克西氏藻）中进入光合作产物并作为碳储存的碳流动
Yoshinori Tsuji,Masatoshi Yamazaki,Iwane Suzuki et al.
Yoshinori Tsuji et al.
The bloom-forming coccolithophore Emiliania huxleyi (Haptophyta) is a dominant marine phytoplankton, cells of which are covered with calcareous plates (coccoliths). E. huxleyi produces unique lipids of C37-C40 long-chain ketones (alkenones)...