Aza-peptide epoxides: potent and selective inhibitors of Schistosoma mansoni and pig kidney legumains (asparaginyl endopeptidases) [0.03%]
aza-肽环氧乙烷类化合物：姜片虫和猪肾半乳糖苷酶（天冬酰胺内切蛋白酶）的有效抑制剂
Karen Ellis James,Marion G Götz,Conor R Caffrey et al.
Karen Ellis James et al.
Aza-peptide epoxides are a new class of irreversible cysteine protease inhibitors. Derivatives containing a P1 aza-asparagine residue are specific for Schistosoma mansoni and pig kidney legumains, which are clan CD cysteine proteases. The i...
Potent bivalent inhibition of human tryptase-beta by a synthetic inhibitor [0.03%]
一种合成抑制剂对人胰蛋白酶β的高效双价抑制作用
Trevor Selwood,Kyle C Elrod,Norman M Schechter
Trevor Selwood
Human tryptase-beta (HTbeta) is a unique serine protease exhibiting a frame-like tetramer structure with four active sites directed toward a central pore. Potent inhibition of HTbeta has been attained using CRA-2059. This compound has two p...
A novel water-soluble and cell-permeable calpain inhibitor protects myocardial and mitochondrial function in postischemic reperfusion [0.03%]
一种新型的水溶性和细胞通透性的钙蛋白酶抑制剂可保护缺血再灌注后的心肌和线粒体功能
Christiane Neuhof,Oliver Götte,Sonata Trumbeckaite et al.
Christiane Neuhof et al.
The effects of the novel calpain inhibitor A-705239 were studied in isolated perfused rabbit hearts subjected to 45 min of global ischemia, followed by 60 min of reperfusion. During 15 min of perfusion the inhibitor accumulated in myocardia...
The binding of haptoglobin to apolipoprotein AI: influence of hemoglobin and concanavalin A [0.03%]
溶血对载脂蛋白AI与血液运送蛋白质结合的影响研究
Maria Stefania Spagnuolo,Luisa Cigliano,Paolo Abrescia
Maria Stefania Spagnuolo
Haptoglobin (Hp) can be purified by affinity chromatography using hemoglobin (Hb)-linked Sepharose. Elution with 8 M urea is generally performed, resulting in heavy contamination of the Hp preparation by apolipoprotein AI (ApoAI), and parti...
Stephan Schilling,Susanne Manhart,Torsten Hoffmann et al.
Stephan Schilling et al.
Glutaminyl cyclases (QC) catalyze the intramolecular cyclization of N-terminal glutamine residues of peptides and proteins. For a comparison of the substrate specificity of human and papaya QC enzymes, a novel continuous assay was establish...
Comparative Study
Biological chemistry. 2003 Dec;384(12):1583-92. DOI:10.1515/BC.2003.175 2003
Anti-mouse GPI-PLD antisera highlight structural differences between murine and bovine GPI-PLDs [0.03%]
抗鼠GPI-PLD抗体揭示了小鼠和牛GPI-PLD结构的差异
Patrick Gregory,Andrew Ziemiecki,Gisela Zürcher et al.
Patrick Gregory et al.
Despite its well characterised biochemistry, the physiological role of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is unknown. Most of the previous studies investigating the distribution of GPI-PLD have focused on the hu...
Comparative Study
Biological chemistry. 2003 Dec;384(12):1575-82. DOI:10.1515/BC.2003.174 2003
Effects of iron limitation on the respiratory chain and the membrane cytochrome pattern of the Euryarchaeon Halobacterium salinarum [0.03%]
铁限制对盐 Haloarchaeon 盐杆菌呼吸链和膜细胞色素模式的影响
Dirk Hubmacher,Berthold F Matzanke,Stefan Anemüller
Dirk Hubmacher
The effects of iron limitation on the electron transport chain of the extremely halophilic Euryarchaeon Halobacterium salinarum were analyzed. When iron was growth-limiting, the respiratory rates as well as the inhibition pattern of the mem...
Characterisation of human dipeptidyl peptidase IV expressed in Pichia pastoris. A structural and mechanistic comparison between the recombinant human and the purified porcine enzyme [0.03%]
人二肽基肽酶Ⅳ在毕赤酵母中的表达及重组人的和猪的二肽基肽酶Ⅳ结构与机理上的比较研究
Joachim Bär,Anja Weber,Torsten Hoffmann et al.
Joachim Bär et al.
Dipeptidyl peptidase IV/CD26 (DP IV) is a multifunctional serine protease cleaving off dipeptides from the N-terminus of peptides. The enzyme is expressed on the surface of epithelial and endothelial cells as a type II transmembrane protein...
Comparative Study
Biological chemistry. 2003 Dec;384(12):1553-63. DOI:10.1515/BC.2003.172 2003
Metabolic stability, receptor binding, cAMP generation, insulin secretion and antihyperglycaemic activity of novel N-terminal Glu9-substituted analogues of glucagon-like peptide-1 [0.03%]
关于一类新型N末端Glu9取代的GLP-1类似物的代谢稳定性、受体结合、cAMP生成、胰岛素分泌及降糖活性的研究
Brian D Green,Victor A Gault,Nigel Irwin et al.
Brian D Green et al.
Glucagon-like peptide-1(7-36)amide (GLP-1) is an incretin hormone with therapeutic potential for type 2 diabetes. Rapid removal of the N-terminal dipeptide, His7-Ala8, by the ubiquitous enzyme dipeptidyl peptidase IV (DPP IV) curtails the b...
Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants [0.03%]
应用于等位基因特异性实时定量PCR提高单核苷酸多态性分型选择性的C4'-烷基化脱氧核糖引物系统(CAPS)
Björn Tews,Jochen Wilhelm,Daniel Summerer et al.
Björn Tews et al.
This study describes a quantitative real-time PCR-based approach for discrimination of single nucleotide sequence variants, called CAPS (C4' alkylated primer system). To increase the discrimination potential of DNA polymerases against compe...
Comparative Study
Biological chemistry. 2003 Oct-Nov;384(10-11):1533-41. DOI:10.1515/BC.2003.170 2003