Human cathepsin F: expression in baculovirus system, characterization and inhibition by protein inhibitors [0.03%]
人源半胱天冬酶F的表达、纯化及丝氨酸蛋白酶抑制剂对它的抑制作用研究
Marko Fonovic,Dieter Brömme,Vito Turk et al.
Marko Fonovic et al.
Recombinant full-length human procathepsin F, produced in the baculovirus expression system, was partially processed during the purification procedure to a form lacking the N-terminal cystatin-like domain and activated with pepsin. Active c...
Protease degradomics: mass spectrometry discovery of protease substrates and the CLIP-CHIP, a dedicated DNA microarray of all human proteases and inhibitors [0.03%]
蛋白酶降解组学：蛋白酶底物的大规模质谱鉴定及其芯片，即所有人类蛋白酶和抑制剂的专用DNA微阵列
Christopher M Overall,Eric M Tam,Reinhild Kappelhoff et al.
Christopher M Overall et al.
The biological role of most proteases in vivo is largely unknown. Therefore, to develop robust techniques to analyze the protease degradome in cells and tissues and to elucidate their substrate degradomes we have developed a dedicated and c...
Accumulation of mini-plasmin in the cerebral capillaries causes vascular invasion of the murine brain by a pneumotropic influenza A virus: implications for influenza-associated encephalopathy [0.03%]
小纤溶酶在脑微血管的蓄积导致肺炎流感病毒侵犯小鼠脑血管：对流感相关性脑病的意义
Dengfu Yao,Ye Chen,Masamichi Kuwajima et al.
Dengfu Yao et al.
The infectivity and pathogenicity of influenza virus are primarily determined by host cellular trypsin-type processing proteases which cleave the viral membrane fusion glycoprotein hemagglutinin (HA). Therefore the distribution of the proce...
Searching for the most effective screening system to identify cell-active inhibitors of beta-secretase [0.03%]
寻找最有效的筛选系统以鉴定针对β-分泌酶的细胞活性抑制剂
Oliver Middendorp,Urs Lüthi,Felix Hausch et al.
Oliver Middendorp et al.
The beta-secretase BACE1 is an attractive drug target for reducing the level of the Alzheimer's disease-promoting Abeta peptide in the brain. Whereas potent peptidomimetic in vitro inhibitors of BACE1 have been designed, screening approache...
Cathepsin L and Arg/Lys aminopeptidase: a distinct prohormone processing pathway for the biosynthesis of peptide neurotransmitters and hormones [0.03%]
猫粮蛋白酶L和Arg / Lys氨基肽酶：肽类神经递质和激素生物合成的独特的前激素加工途径
Vivian Hook,Sukkid Yasothornsrikul,Doron Greenbaum et al.
Vivian Hook et al.
Peptide neurotransmitters and hormones are synthesized as protein precursors that require proteolytic processing to generate smaller, biologically active peptides that are secreted to mediate neurotransmission and hormone actions. Neuropept...
Amanda Wendt,Valery F Thompson,Darrel E Goll
Amanda Wendt
Calpastatin is a multiheaded inhibitor capable of inhibiting more than one calpain molecule. Each inhibitory domain of calpastatin has three subdomains, A, B, and C; A binds to domain IV and C binds to domain VI of the calpains. Crystallogr...
Charleen Rupnarain,Zodwa Dlamini,Sarala Naicker et al.
Charleen Rupnarain et al.
Colon cancer is the third most common cancer globally. The risk of developing colon cancer is influenced by a number of factors that include age and diet, but is primarily a genetic disease, resulting from oncogene over-expression and tumou...
Cathepsins K, L, B, X and W are differentially expressed in normal and chronically inflamed gastric mucosa [0.03%]
组织化学；猫肉蛋白酶K、L、B、X和W在正常胃黏膜与慢性炎症胃黏膜中的表达差异研究
Frank Bühling,Ulrich Peitz,Sabine Krüger et al.
Frank Bühling et al.
The expression of cathepsins K, L, B, X and W was studied by quantitative RT-PCR in normal and inflamed gastric mucosa (antrum, corpus, cardia). Cathepsins B, L, K and X were expressed ubiquitously. In contrast, cathepsin W was expressed at...
Synthesis and antiplasmodial activity of a cysteine protease-inhibiting biotinylated aziridine-2,3-dicarboxylate [0.03%]
一种用于生物素化氮杂环氧乙烷-2，3-二甲酸酯的半胱氨酸蛋白酶抑制剂的合成与抗疟活性分析
Christoph Gelhaus,Radim Vicik,Rolf Hilgenfeld et al.
Christoph Gelhaus et al.
Cysteine proteases have been implicated in a variety of processes essential for the survival and progression of the malarial parasite Plasmodium falciparum. Here, we synthesized a cysteine protease inhibitor that contains the electrophilic ...
New protease inhibitors from buckwheat seeds: properties, partial amino acid sequences and possible biological role [0.03%]
新的荞麦种子蛋白酶抑制剂的性质、部分氨基酸序列及其可能的生物学作用
Tatiana Tsybina,Yakov Dunaevsky,Alexander Musolyamov et al.
Tatiana Tsybina et al.
Preparations of new low molecular weight protein inhibitors of serine proteinases have been obtained from buckwheat Fagopyrum esculentum seeds by chromatography of seed extracts on trypsin-Sepharose 4B, Mono-Q and Mono-S ion-exchangers. The...