Homology modeling and SAR analysis of Schistosoma japonicum cathepsin D (SjCD) with statin inhibitors identify a unique active site steric barrier with potential for the design of specific inhibitors [0.03%]
基于他汀类抑制剂对中国血吸虫半胱氨酸蛋白酶D（SjCD）的同源模建及构效关系分析，发现一个独特的活性位点空间屏障并为进一步设计其特异性抑制剂提供了可能
Conor R Caffrey,Lenka Placha,Cyril Barinka et al.
Conor R Caffrey et al.
Proteases that digest the blood-meal of the parasitic fluke Schistosoma are potential targets for therapy of schistosomiasis, a disease of chronic morbidity in humans. We generated a three-dimensional model of the cathepsin D target proteas...
Hydrogen peroxide causes greater oxidation in cellular RNA than in DNA [0.03%]
过氧化氢引起的细胞内RNA氧化程度大于DNA
Tim Hofer,Carine Badouard,Edyta Bajak et al.
Tim Hofer et al.
Human A549 lung epithelial cells were challenged with 18O-labeled hydrogen peroxide ([18O]-H2O2), the total RNA and DNA extracted in parallel, and analyzed for 18O-labeled 8-oxo-7,8-dihydroguanosine ([18O]-8-oxoGuo) and 8-oxo-7,8-dihydro-2'...
Comparative Study
Biological chemistry. 2005 Apr;386(4):333-7. DOI:10.1515/BC.2005.040 2005
Véronique Cruciani,Svein-Ole Mikalsen
Véronique Cruciani
Unannotated mammalian genome databases (dog, cow, opossum) were searched for candidate connexin genes, using sequences from annotated genomes (man, mouse). All 18 'multi-species' connexin genes, i.e., orthologs of connexin26 , 29/31.3 (dupl...
Identification of three novel mutations in the dihydropyrimidine dehydrogenase gene associated with altered pre-mRNA splicing or protein function [0.03%]
三种与改变的前信使核糖核酸剪接或蛋白质功能相关的胸苷酸合成酶基因新型突变的鉴定
André B P Van Kuilenburg,Rutger Meinsma,Eva Beke et al.
André B P Van Kuilenburg et al.
Dihydropyrimidine dehydrogenase (DPD) is the initial and rate-limiting enzyme in the catabolism of the pyrimidine bases uracil and thymine, as well as of the widely used chemotherapeutic drug 5-fluorouracil (5FU). Analysis of the DPD gene (...
Nicking activity on pBR322 DNA of ribosome inactivating proteins from Phytolacca dioica L. leaves [0.03%]
Phytolacca dioica L.叶中核糖体失活蛋白对pBR322 DNA的切刻作用
Serena Aceto,Antimo Di Maro,Barbara Conforto et al.
Serena Aceto et al.
Ribosome-inactivating proteins isolated from Phytolacca dioica L. leaves are rRNA-N-glycosidases, as well as adenine polynucleotide glycosylases. Here we report that some of them cleave supercoiled pBR322 dsDNA, generating relaxed and linea...
A new selective substrate for cathepsin E based on the cleavage site sequence of alpha2-macroglobulin [0.03%]
一种基于α₂-巨球蛋白裂解位点序列的针对猫肉毒菌素E的选择性底物
Yoshiyuki Yasuda,Keiko Kohmura,Tomoko Kadowaki et al.
Yoshiyuki Yasuda et al.
Cathepsin E is an intracellular aspartic proteinase of the pepsin family predominantly expressed in cells of the immune system and believed to contribute to homeostasis by participating in host defense mechanisms. Studies on its enzymatic p...
Enzymatic profiling of human kallikrein 14 using phage-display substrate technology [0.03%]
人激肽原酶14的酶活性底物序列及表型分析
Loyse M Felber,Carla A Borgoño,Sylvain M Cloutier et al.
Loyse M Felber et al.
The human KLK14 gene is one of the newly identified serine protease genes belonging to the human kallikrein family, which contains 15 members. KLK14 , like all other members of the human kallikrein family, is predicted to encode for a secre...
One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase [0.03%]
视杆激酶与recoverin之间相互作用的钙调机制
Konstantin E Komolov,Dimitri V Zinchenko,Valeriya A Churumova et al.
Konstantin E Komolov et al.
Recoverin is a neuronal calcium sensor protein that controls the activity of rhodopsin kinase in a Ca(2+)-dependent manner. Mutations in the EF-hand Ca2+ binding sites are valuable tools for investigating the functional properties of recove...
Jutta Ludwig-Müller,Sergey V Tokalov,Alexander Franz et al.
Jutta Ludwig-Müller et al.
The metabolism of the flavonol quercetin in human leukaemia (HL-60) cells was investigated. The fluorescence that is elicited by quercetin upon binding to a target protein was quickly attenuated in vital cells, while apoptotic cells showed ...
Trehalose and 6-aminohexanoic acid stabilize and renature glucose-6-phosphate dehydrogenase inactivated by glycation and by guanidinium hydrochloride [0.03%]
海藻糖和己内酰胺酸稳定并复原葡萄糖-6-磷酸脱氢酶（此酶此前已甘化处理或用盐酸胍变性）
Elena Ganea,John J Harding
Elena Ganea
A number of naturally occurring small organic molecules, primarily involved in maintaining osmotic pressure in the cell, display chaperone-like activity, stabilizing the native conformation of proteins and protecting them from various kinds...