Proinsulin lacking the A7-B7 disulfide bond, Ins2Akita, tends to aggregate due to the exposed hydrophobic surface [0.03%]
Takeo Yoshinaga,Keisuke Nakatome,Jun-ichi Nozaki et al.
Takeo Yoshinaga et al.
A single mutation (C96Y) in the Ins2 gene, which disrupts the A7-B7 disulfide bond, causes the diabetic phenotype in Akita mice. We biochemically analyzed the conformation of wild-type and Akita mutant recombinant proinsulins. Gel filtratio...
Degradation of the sodium taurocholate cotransporting polypeptide (NTCP) by the ubiquitin-proteasome system [0.03%]
钠 taurocholate 同转运多肽（NTCP）通过泛素-蛋白酶体系统降解
Thomas Kühlkamp,Verena Keitel,Angelika Helmer et al.
Thomas Kühlkamp et al.
The sodium taurocholate cotransporting polypeptide (Ntcp, Slc10a1) is the major uptake system for bile acids into liver cells. This study investigated the degradation of rat Ntcp and human NTCP by the ubiquitin-proteasome system (UPS). In s...
Impact of Mycoplasma hyorhinis infection on L-arginine metabolism: differential regulation of the human and murine iNOS gene [0.03%]
Mycoplasma hyorhinis感染对L-精氨酸代谢的影响：人类和鼠iNOS基因的差异调节
Guido Kagemann,Birgit Henrich,Michaela Kuhn et al.
Guido Kagemann et al.
Infection with mycoplasma is a common problem in cell cultures, with Mycoplasma hyorhinis being the predominant species. Here we investigate the effect of M. hyorhinis infection on L-arginine metabolism, with focus on iNOS-mediated NO synth...
Comparative Study
Biological chemistry. 2005 Oct;386(10):1055-63. DOI:10.1515/BC.2005.121 2005
Spectroscopic characterization of the iron-oxo intermediate in cytochrome P450 [0.03%]
细胞色素P450铁氧中间体的光谱表征
Christiane Jung,Volker Schünemann,Friedhelm Lendzian et al.
Christiane Jung et al.
From analogy to chloroperoxidase from Caldariomyces fumago, it is believed that the electronic structure of the intermediate iron-oxo species in the catalytic cycle of cytochrome P450 corresponds to an iron(IV) porphyrin-pi-cation radical (...
Comparative Study
Biological chemistry. 2005 Oct;386(10):1043-53. DOI:10.1515/BC.2005.120 2005
Albrecht Berkessel,Magali Dousset,Stephan Bulat et al.
Albrecht Berkessel et al.
The copper enzyme galactose oxidase (GOase, EC 1.1.3.9) catalyses the oxidation of D-galactose and other primary alcohols in air to the corresponding aldehydes and hydrogen peroxide. The current mechanistic hypothesis for this two-electron ...
Biomimetic metal-radical reactivity: aerial oxidation of alcohols, amines, aminophenols and catechols catalyzed by transition metal complexes [0.03%]
仿生金属自由基反应：过渡金属配合物催化醇、胺、氨基酚和儿茶酚的氧化反应
Phalguni Chaudhuri,Karl Wieghardt,Thomas Weyhermüller et al.
Phalguni Chaudhuri et al.
The contributions of the authors to the research program 'Radicals in Enzymatic Catalysis' over the last ca. 5 years are summarized. Significant efforts were directed towards the design and testing of phenol-containing ligands for synthesiz...
Spectroscopic and theoretical approaches for studying radical reactions in class I ribonucleotide reductase [0.03%]
光谱学和理论研究方法在I类核糖核苷酸还原酶自由基反应中的应用
Marina Bennati,Friedhelm Lendzian,Michael Schmittel et al.
Marina Bennati et al.
Ribonucleotide reductases (RNRs) catalyze the production of deoxyribonucleotides, which are essential for DNA synthesis and repair in all organisms. The three currently known classes of RNRs are postulated to utilize a similar mechanism for...
Novel bacterial molybdenum and tungsten enzymes: three-dimensional structure, spectroscopy, and reaction mechanism [0.03%]
新颖的细菌钼和钨酶：三维结构、光谱学及反应机理
Matthias Boll,Bernhard Schink,Albrecht Messerschmidt et al.
Matthias Boll et al.
The molybdenum enzymes 4-hydroxybenzoyl-CoA reductase and pyrogallol-phloroglucinol transhydroxylase and the tungsten enzyme acetylene hydratase catalyze reductive dehydroxylation reactions, i.e., transhydroxylation between phenolic residue...
Matthias Boll,Georg Fuchs
Matthias Boll
Aerobic bacteria use molecular oxygen as a common co-substrate for key enzymes of aromatic metabolism. In contrast, in anaerobes all oxygen-dependent reactions are replaced by a set of alternative enzymatic processes. The anaerobic degradat...
New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria [0.03%]
新型甘氨双键酶催化厌氧菌中的关键代谢步骤
Thorsten Selmer,Antonio J Pierik,Johann Heider
Thorsten Selmer
During the last decade, an increasing number of new enzymes containing glycyl radicals in their active sites have been identified and biochemically characterised. These include benzylsuccinate synthase (Bss), 4-hydroxyphenylacetate decarbox...