6S RNA - an ancient regulator of bacterial RNA polymerase rediscovered [0.03%]
6S RNA——这一古老的细菌RNA聚合酶调控因子再发现
Dagmar K Willkomm,Roland K Hartmann
Dagmar K Willkomm
The bacterial riboregulator 6S RNA was one of the first non-coding RNAs to be discovered in the late 1960s, but its cellular role remained enigmatic until the year 2000. 6S RNA, only recognized to be ubiquitous among bacteria in 2005, binds...
Alternative pre-mRNA splicing in the human system: unexpected role of repetitive sequences as regulatory elements [0.03%]
人类系统中前信使核糖核酸的选择性剪接：重复序列作为调控元件的意外作用
Jingyi Hui,Albrecht Bindereif
Jingyi Hui
Alternative splicing is a process by which multiple messenger RNAs (mRNAs) are generated from a single pre-mRNA, resulting in functionally distinct protein products. This is accomplished by the differential recognition of splice sites in th...
The tRNase Z family of proteins: physiological functions, substrate specificity and structural properties [0.03%]
tRNase Z蛋白家族：生理功能、底物特异性和结构性质
Andreas Vogel,Oliver Schilling,Bettina Späth et al.
Andreas Vogel et al.
tRNase Z is the endoribonuclease that generates the mature 3'-end of tRNA molecules by removal of the 3'-trailer elements of precursor tRNAs. This enzyme has been characterized from representatives of all three domains of life (Bacteria, Ar...
Species-specific antibiotic-ribosome interactions: implications for drug development [0.03%]
抗生素与核糖体的物种特异性相互作用及其对药物开发的影响
Daniel N Wilson,Jörg M Harms,Knud H Nierhaus et al.
Daniel N Wilson et al.
In the cell, the protein synthetic machinery is a highly complex apparatus that offers many potential sites for functional interference and therefore represents a major target for antibiotics. The recent plethora of crystal structures of ri...
Jörg Vogel,Cynthia Mira Sharma
Jörg Vogel
Small non-coding RNAs (sRNAs) have attracted considerable attention as an emerging class of gene expression regulators. In bacteria, a few regulatory RNA molecules have long been known, but the extent of their role in the cell was not fully...
Po Yu Chen,Gunter Meister
Po Yu Chen
microRNAs (miRNAs) form an evolutionarily conserved and highly abundant class of non-coding RNAs that are 21-24 nucleotides (nt) in length. They are processed from double-stranded (ds) RNA precursors and sequence-specifically guide posttran...
Highlight: RNA biochemistry [0.03%]
聚焦核糖核酸生化
Anita Marchfelder,Roland K Hartmann
Anita Marchfelder
Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects [0.03%]
Elmar A Siess,Monika M Hofstetter
Elmar A Siess
A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on ...
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L [0.03%]
Luciano Puzer,Simone S Cotrin,Maria H S Cezari et al.
Luciano Puzer et al.
The S1 and S2 subsite specificity of recombinant human cathepsins X was studied using fluorescence resonance energy transfer (FRET) peptides with the general sequences Abz-Phe-Xaa-Lys(Dnp)-OH and Abz-Xaa-Arg-Lys(Dnp)-OH, respectively (Abz=o...
Comparative Study
Biological chemistry. 2005 Nov;386(11):1191-5. DOI:10.1515/BC.2005.136 2005
Adriana F de Souza,Ricardo J S Torquato,Aparecida S Tanaka et al.
Adriana F de Souza et al.
A Bauhinia variegata trypsin inhibitor (BvTI) cDNA fragment was cloned into the pCANTAB5E phagemid. The clone pAS 1.1.3 presented a cDNA fragment of 733 bp, including the coding region for a mature BvTI protein comprising 175 amino acid res...