Idiosyncratic cleavage and ligation activity of individual hammerhead ribozymes and core sequence variants thereof [0.03%]
个别锤头核酶及核心序列变异体的特异性切割和连接活性
Rita Przybilski,Christian Hammann
Rita Przybilski
The hammerhead ribozyme is a small RNA endonuclease found in sub-viral plant pathogens, in transcripts from certain animal satellite DNAs and encoded at distinct loci of Arabidopsis thaliana. Kinetic analyses of tertiary stabilised ribozyme...
Morphing the minimal and full-length hammerhead ribozymes: implications for the cleavage mechanism [0.03%]
锤头核酶的最小结构与全长结构的比较及对剪切机理的影响
William G Scott
William G Scott
The hammerhead ribozyme is a small, intensively studied catalytic RNA, and has been used as a prototype for understanding how RNA catalysis works. In 2003, the importance of a set of tertiary contacts that appear in natural sequences of the...
A 2'-methyl or 2'-methylene group at G+1 in precursor tRNA interferes with Mg2+ binding at the enzyme-substrate interface in E-S complexes of E. coli RNase P [0.03%]
在大肠杆菌RNase P的酶底物复合物中，预tRNA的G+1上带有2'-甲基或2'-亚甲基团会干扰Mg2+结合
Simona Cuzic,Roland K Hartmann
Simona Cuzic
We analyzed processing of precursor tRNAs carrying a single 2'-deoxy, 2'-OCH(3), or locked nucleic acid (LNA) modification at G+1 by Escherichia coli RNase P RNA in the absence and presence of its protein cofactor. The extra methyl or methy...
Long-range impact of peripheral joining elements on structure and function of the hepatitis delta virus ribozyme [0.03%]
外周接合元件对乙肝病毒基因组ribozyme结构与功能的远程影响
Rebecca A Tinsley,Nils G Walter
Rebecca A Tinsley
The HDV ribozyme is an RNA enzyme from the human pathogenic hepatitis delta virus (HDV) that has recently also been identified in the human genome. It folds into a compact, nested double-pseudoknot. We examined here the functional relevance...
A chemo-genetic approach for the study of nucleobase participation in nucleolytic ribozymes [0.03%]
核酸酶核糖核酸酶中核苷酸参与的化学遗传学方法研究
David M J Lilley
David M J Lilley
A novel chemo-genetic approach for the analysis of general acid-base catalysis by nucleobases in ribozymes is reviewed. This involves substitution of a C-nucleoside with imidazole in place of a natural nucleobase. The Varkud satellite riboz...
Saba Valadkhan
Saba Valadkhan
The spliceosome, the multi-megadalton molecular machine that performs splicing, consists of over 200 different proteins and five small nuclear RNAs (snRNAs). Extensive mechanistic and structural similarities to self-splicing group II intron...
Importance of tRNA interactions with 23S rRNA for peptide bond formation on the ribosome: studies with substrate analogs [0.03%]
tRNA与23S rRNA相互作用对于核糖体上以底物类似物为底物的肽键形成的重要性：采用底物类似物的研究
Malte Beringer,Marina V Rodnina
Malte Beringer
The major enzymatic activity of the ribosome is the catalysis of peptide bond formation. The active site -- the peptidyl transferase center -- is composed of ribosomal RNA (rRNA), and interactions between rRNA and the reactants, peptidyl-tR...
Steinar D Johansen,Peik Haugen,Henrik Nielsen
Steinar D Johansen
Ribosomal DNA (rDNA) is a specialised chromosomal location that is dedicated to high-level transcription of ribosomal RNA genes. Interestingly, rDNAs are frequently interrupted by parasitic elements, some of which carry protein genes. These...
Olga Fedorova,Nora Zingler
Olga Fedorova
Group II introns are large autocatalytic RNAs found in organellar genomes of plants and lower eukaryotes, as well as in some bacterial genomes. Interestingly, these ribozymes share characteristic traits with both spliceosomal introns and no...
Sidney Altman
Sidney Altman