Differential effects of novel tumour-derived p53 mutations on the transformation of NIH-3T3 cells [0.03%]
新肿瘤衍生的p53突变对NIH-3T3细胞转化的不同影响
Howard Donninger,Anke Binder,Lothar Bohm et al.
Howard Donninger et al.
The p53 tumour suppressor gene is frequently mutated in human tumours and different tumour-derived mutations have varying effects on cells. The effect of a novel tumour-derived p53 mutation and two recently described mutations from South Af...
Collagen IV regulates Caco-2 cell spreading and p130Cas phosphorylation by FAK-dependent and FAK-independent pathways [0.03%]
IV型胶原通过FAK依赖和非依赖途径调节Caco-2细胞的伸展及p130Cas磷酸化
Matthew A Sanders,Marc D Basson
Matthew A Sanders
We previously observed that collagen IV regulates Caco-2 intestinal epithelial cell spreading and migration via Src-dependent p130(Cas) phosphorylation and stimulates focal adhesion kinase (FAK). However, the role of FAK and the related kin...
Synthesis and characterization of a functional intact IgG in a prokaryotic cell-free expression system [0.03%]
在原核无细胞表达系统中全长功能性IgG的合成与表征
Stephan Frey,Martin Haslbeck,Otmar Hainzl et al.
Stephan Frey et al.
Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged p...
Birendra Singh,Klaus-Heinrich Röhm
Birendra Singh
The specificity of bacterial nutrient importers of the ATP-binding cassette (ABC) type depends on external receptor proteins that not only bind the solute to be transported, but also initiate the transport process by inducing ATP hydrolysis...
Cloning, functional analysis, and mitochondrial localization of Trypanosoma brucei monothiol glutaredoxin-1 [0.03%]
巴贝虫单硫型谷氧还蛋白1的克隆、功能分析及线粒体定位
Michael Filser,Marcelo A Comini,Maria M Molina-Navarro et al.
Michael Filser et al.
African trypanosomes encode three monothiol glutaredoxins (1-C-Grx1 to 3). 1-C-Grx1 has a putative CAYS active site and Cys181 as single additional cysteine. The recombinant protein forms non-covalent homodimers. As observed for other monot...
Reassessing the role of a 3'-UTR-binding translational inhibitor in regulation of circadian bioluminescence rhythm in the dinoflagellate Gonyaulax [0.03%]
甲藻腔腕藻生物发光节律调控中3 '- UTR结合翻译抑制因子的作用再评估研究
Mathieu Lapointe,David Morse
Mathieu Lapointe
The nightly bioluminescence of the dinoflagellate Gonyaulax is a circadian rhythm caused by the presence in cells of specialized bioluminescent organelles, termed scintillons, containing the reaction catalyst luciferase, the substrate lucif...
A counterintuitive approach to treat enzyme deficiencies: use of enzyme inhibitors for restoring mutant enzyme activity [0.03%]
治疗酶缺乏的反直觉方法：使用酶抑制剂恢复突变酶活性
Jian-Qiang Fan
Jian-Qiang Fan
Pharmacological chaperone therapy is an emerging counterintuitive approach to treat protein deficiencies resulting from mutations causing misfolded protein conformations. Active-site-specific chaperones (ASSCs) are enzyme active-site direct...
Macrophage paraoxonase 2 (PON2) expression is upregulated by unesterified cholesterol through activation of the phosphatidylinositol 3-kinase (PI3K) pathway [0.03%]
未酯化胆固醇通过激活磷脂酰肌醇3-激酶信号途径诱导巨噬细胞PON2表达上调
Maayan Shiner,Bianca Fuhrman,Michael Aviram
Maayan Shiner
Advanced atherosclerotic lesions are characterized by a progressive increase in the unesterified cholesterol (UC) content and a decrease in its cholesteryl ester (CE) content. In the present study, we examined mechanisms involved in the eff...
Ultraviolet B radiation induces cell shrinkage and increases osmolyte transporter mRNA expression and osmolyte uptake in HaCaT keratinocytes [0.03%]
紫外线B辐射诱导角质形成细胞HaCaT发生胞体萎缩以及渗透调节物转运蛋白mRNA表达和渗透调节物摄取增加的现象
Ulrich Warskulat,Stefanie Brookmann,Andrea Reinen et al.
Ulrich Warskulat et al.
We have previously shown that compatible organic osmolytes, such as betaine, myo-inositol and taurine, are part of the stress response of normal human keratinocytes (NHKs) to ultraviolet B (UVB) radiation. In this regard, we tested human Ha...
Overexpression and mass spectrometry analysis of mature human acid ceramidase [0.03%]
人成熟酸性类固醇酶的过表达及质谱分析
Heike Schulze,Ute Schepers,Konrad Sandhoff
Heike Schulze
Human acid ceramidase catalyzes the last step of lysosomal sphingolipid degradation, the hydrolysis of ceramide to sphingosine and free fatty acid. Inherited deficiency of acid ceramidase activity leads to Farber disease (Farber lipogranulo...