Biocatalytic properties of a novel crude glycyrrhizin hydrolase from the liver of the domestic duck [0.03%]
家鸭肝脏中粗甘草酸水解酶的生物催化性质
Ding-Qiang Lu,Hui Li,Yan Dai et al.
Ding-Qiang Lu et al.
A novel crude glycyrrhizin (GL) hydrolase preparation from the liver of domestic duck was used to produce glycyrrhetic acid monoglucuronide. To characterize the biocatalytic profiles of the crude enzyme, some effect factors were investigate...
Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase from Ralstonia eutropha [0.03%]
缺乏辅酶A的活性、晶体结构及 Rational 工程的多功能β-酮脂酰硫酯酶（来自Ralstonia eutropha）
Christopher D Fage,Jessica L Meinke,Adrian T Keatinge-Clay
Christopher D Fage
Thiolases catalyze the formation of carbon-carbon bonds in diverse biosynthetic pathways. The promiscuous β-ketoacyl thiolase B of Ralstonia eutropha (ReBktB) has been utilized in the in vivo conversion of Coenzyme A (CoA)-linked precursor...
Yihx-encoded haloacid dehalogenase-like phosphatase HAD4 from Escherichia coli is a specific α-d-glucose 1-phosphate hydrolase useful for substrate-selective sugar phosphate transformations [0.03%]
大肠杆菌YihX编码的Haloacid脱卤酶样磷酸酶HAD4是一种特异性α-D-葡萄糖-1-磷酸水解酶，可应用于底物选择性糖磷酸反应
Martin Pfeiffer,Patricia Wildberger,Bernd Nidetzky
Martin Pfeiffer
Phosphomonoester hydrolases (phosphatases; EC 3.1.3.) often exhibit extremely relaxed substrate specificity which limits their application to substrate-selective biotransformations. In search of a phosphatase catalyst specific for hydrolyzi...
Exploring nicotinamide cofactor promiscuity in NAD(P)H-dependent flavin containing monooxygenases (FMOs) using natural variation within the phosphate binding loop. Structure and activity of FMOs from Cellvibrio sp. BR and Pseudomonas stutzeri NF13 [0.03%]
探索NAD(P) H依赖型黄素单加氧酶（FMO）中的烟酰胺辅因子的多功能性：使用磷酸结合环内的自然差异，以及来自BR和NF13种Cellvibrio和Pseudomonas stutzeri的FMOs的结构与活性
Chantel N Jensen,Sohail T Ali,Michael J Allen et al.
Chantel N Jensen et al.
Flavin-containing monooxygenases (FMOs) catalyse asymmetric oxidation reactions that have potential for preparative organic synthesis, but most use the more expensive, phosphorylated nicotinamide cofactor NADPH to reduce FAD to FADH2 prior ...
Investigation of the donor and acceptor range for chiral carboligation catalyzed by the E1 component of the 2-oxoglutarate dehydrogenase complex [0.03%]
研究2-氧代戊二酸脱氢酶大亚基E1催化手性卡巴利贡反应的亲核剂和活化剂底物范围
Hetalben Patel,Da Jeong Shim,Edgardo T Farinas et al.
Hetalben Patel et al.
The potential of thiamin diphosphate (ThDP)-dependent enzymes to catalyze C-C bond forming (carboligase) reactions with high enantiomeric excess has been recognized for many years. Here we report the application of the E1 component of the E...
Microfluidic multi-input reactor for biocatalytic synthesis using transketolase [0.03%]
用于转酮酶生物催化的微流体多输入反应器
James Lawrence,Brian O&#x;Sullivan,Gary J Lye et al.
James Lawrence et al.
Biocatalytic synthesis in continuous-flow microreactors is of increasing interest for the production of specialty chemicals. However, the yield of production achievable in these reactors can be limited by the adverse effects of high substra...
Calculation of substrate binding affinities for a bacterial GH78 rhamnosidase through molecular dynamics simulations [0.03%]
分子动力学模拟计算细菌型GH78半乳糖醛酸糖苷酶底物结合亲和力
Melanie Grandits,Herbert Michlmayr,Christoph Sygmund et al.
Melanie Grandits et al.
Ram2 from Pediococcus acidilactici is a rhamnosidase from the glycoside hydrolase family 78. It shows remarkable selectivity for rutinose rather than para-nitrophenyl-alpha-l-rhamnopyranoside (p-NPR). Molecular dynamics simulations were per...
Amino ester hydrolase from Xanthomonas campestris pv. campestris, ATCC 33913 for enzymatic synthesis of ampicillin [0.03%]
来自伤寒沙门氏菌的氨基酸酯酶水解物用于 Ampicillin 的合成反应
Janna K Blum,Andreas S Bommarius
Janna K Blum
α-Amino ester hydrolases (AEH) are a small class of proteins, which are highly specific for hydrolysis or synthesis of α-amino containing amides and esters including β-lactam antibiotics such as ampicillin, amoxicillin, and cephalexin. A...
Hua Zhao,Gary A Baker,Shaletha Holmes
Hua Zhao
Deep eutectic solvents (DESs) consisting of mixtures of a choline salt (chloride or acetate form) and glycerol are prepared as easily accessible, biodegradable, and inexpensive alternatives to conventional aprotic cation-anion paired ionic ...
Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg [0.03%]
长时间接触有机溶剂对羧肽酶Carlsberg活性部位环境的影响
Vibha Bansal,Yamixa Delgado,Ezio Fasoli et al.
Vibha Bansal et al.
The potential of enzyme catalysis as a tool for organic synthesis is nowadays indisputable, as is the fact that organic solvents affect an enzyme's activity, selectivity and stability. Moreover, it was recently realized that an enzyme's ini...