A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody [0.03%]
一种新的免疫组化染色方法能够在保存抗体的同时快速检测淋巴结微转移灶
Hiroshi Toda,Yoshihiro Minamiya,Masami Kagaya et al.
Hiroshi Toda et al.
We developed a novel ultrarapid immunohistochemical staining method in which an AC electric field is used to facilitate detection of tumor cells. Frozen sections of non-small cell lung cancer in lymph nodes were fixed in acetone for 2 min, ...
Enhanced Autophagy and Reduced Expression of Cathepsin D Are Related to Autophagic Cell Death in Epstein-Barr Virus-Associated Nasal Natural Killer/T-Cell Lymphomas: An Immunohistochemical Analysis of Beclin-1, LC3, Mitochondria (AE-1), and Cathepsin D in Nasopharyngeal Lymphomas [0.03%]
贝纳尔病毒相关鼻自然杀伤/T细胞淋巴瘤中增强的自噬和猫唾液酸酶D表达降低与自噬性细胞死亡有关：鼻咽淋巴瘤中Beclin-1、LC3、线粒体(AE-1)和猫唾液酸酶D的免疫组化分析
Kazuhisa Hasui,Jia Wang,Xinshan Jia et al.
Kazuhisa Hasui et al.
This study investigated autophagy in 37 cases of nasopharyngeal lymphomas including 23 nasal natural killer (NK)/T-cell lymphomas (NKTCL), 3 cytotoxic T-cell lymphomas (cytotoxic-TML) and 9 B-cell lymphomas (BML) by means of antigen-retriev...
Analysis of mTOR inhibition-involved pathway in ovarian clear cell adenocarcinoma [0.03%]
卵巢透明细胞腺癌中mTOR抑制相关信号通路分析研究
Makiko Harasawa,Masanori Yasuda,Takeshi Hirasawa et al.
Makiko Harasawa et al.
This study was designed to clarify the mechanism of the mammalian target of rapamycin (mTOR)-hypoxia inducible factor-1 (HIF-1) pathway using the cultured cell strain derived from human ovarian clear cell adenocarcinoma (CCA). Everolimus (a...
Protein kinase B (akt) promotes pathological angiogenesis in murine model of oxygen-induced retinopathy [0.03%]
蛋白激酶B（Akt）在缺氧诱导性视网膜病变小鼠模型中促进病理性的血管生成
Peng Wang,Xiao-Feng Tian,Jun-Bo Rong et al.
Peng Wang et al.
Akt, or protein kinase B, is an important signaling molecule that modulates many cellular processes such as cell growth, survival, and metabolism. However, the vivo roles and effectors of Akt in retinal angiogenesis are not explicitly clear...
Roles of fukutin, the gene responsible for fukuyama-type congenital muscular dystrophy, in neurons: possible involvement in synaptic function and neuronal migration [0.03%]
富田基因在神经元中的作用：可能参与突触功能和神经元迁移
Atsuko Hiroi,Tomoko Yamamoto,Noriyuki Shibata et al.
Atsuko Hiroi et al.
Fukutin is a gene responsible for Fukuyama-type congenital muscular dystrophy (FCMD), accompanying ocular and brain malformations represented by cobblestone lissencephaly. Fukutin is related to basement membrane formation via the glycosylat...
Histological study and LYVE-1 immunolocalization of mouse mesenteric lymph nodes with "In Vivo Cryotechnique" [0.03%]
活体冷冻制样小鼠肠系淋巴结的组织学与LYVE-1免疫定位研究
Yuqin Bai,Bao Wu,Nobuo Terada et al.
Yuqin Bai et al.
The "in vivo cryotechnique" (IVCT) is a powerful tool to directly freeze living animal organs in order to maintain biological components in frozen tissues, reflecting their native states. In this study, mesenteric lymph nodes of living mice...
PGC-1α mRNA level and oxidative capacity of the plantaris muscle in rats with metabolic syndrome, hypertension, and type 2 diabetes [0.03%]
代谢综合征、高血压和2型糖尿病大鼠比目鱼肌PGC-1αmRNA水平及氧化能力的影响
Fumiko Nagatomo,Hidemi Fujino,Hiroyo Kondo et al.
Fumiko Nagatomo et al.
We examined the fiber profiles and the mRNA levels of peroxisome proliferator-activated receptors (PPARα and PPARδ/β) and of the PPARγ coactivator-1α (PGC-1α) in the plantaris muscles of 15-week-old control (WR), metabolic syndrome (C...
Immunohistochemical distribution of serum proteins in living mouse heart with In vivo cryotechnique [0.03%]
活体内切技术在小鼠心脏中检测血清蛋白的免疫组化分布研究
Liye Shi,Nobuo Terada,Yurika Saitoh et al.
Liye Shi et al.
In vivo cryotechnique (IVCT), which immediately cryofixes target organs in situ, was used to clarify the morphological features of beating heart tissue of living mice. IVCT was performed for diastolic heart tissue under the condition of mon...
Hideyuki Arai,Akira Furusu,Tomoya Nishino et al.
Hideyuki Arai et al.
Thalidomide is clinically recognized as a therapeutic agent for multiple myeloma and has been known to exert anti-angiogenic actions. Recent studies have suggested the involvement of angiogenesis in the progression of peritoneal fibrosis. T...
Characterization of fiber types in different muscles of the hindlimb in female weanling and adult Wistar rats [0.03%]
断乳和成年Wistar大鼠后肢不同肌肉纤维类型的特征研究
Anabelle Silva Cornachione,Priscila Cação Oliveira Benedini-Elias,Juliana Cristina Polizello et al.
Anabelle Silva Cornachione et al.
We analyzed lesser diameter and distribution of fiber types in different skeletal muscles from female Wistar rats using a histoenzymology Myofibrillar Adenosine Tri-phosphatase (mATPase) method. Fragments from muscles were frozen and proces...