Tetsuro Izumi
Tetsuro Izumi
The monomeric GTPase Rab27 regulates exocytosis of a broad range of vesicles in multicellular organisms. Several effectors bind GTP-bound Rab27a and/or Rab27b on secretory vesicles to execute a series of exocytic steps, such as vesicle matu...
Ilkin Aygün,Takashi S Miki
Ilkin Aygün
XRN2 is a 5'-to-3' exoribonuclease that is predominantly localized in the nucleus. By degrading or trimming various classes of RNA, XRN2 contributes to essential processes in gene expression such as transcription termination and ribosome bi...
Hrd1-dependent Degradation of the Unassembled PIGK Subunit of the GPI Transamidase Complex [0.03%]
HRD1依赖的GPI连接酶复合物未组装的PIGK亚基的降解
Kohei Kawaguchi,Miki Yamamoto-Hino,Yoshiko Murakami et al.
Kohei Kawaguchi et al.
Glycosylphosphatidylinositol (GPI)-anchored proteins are post-transcriptionally modified with GPI and anchored to the plasma membrane. GPI is attached to nascent proteins in the endoplasmic reticulum by the GPI transamidase complex, which c...
Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains [0.03%]
GFP敲入C. elegans品系中所有微管蛋白异构体的表达模式及水平分析
Kei Nishida,Kenta Tsuchiya,Hiroyuki Obinata et al.
Kei Nishida et al.
Most organisms have multiple α- and β-tubulin isotypes that likely contribute to the diversity of microtubule (MT) functions. To understand the functional differences of tubulin isotypes in Caenorhabditis elegans, which has nine α-tubuli...
Multiple Ways for Stress Sensing and Regulation of the Endoplasmic Reticulum-stress Sensors [0.03%]
内质网应力传感器的多种应激感应及调节方式
Quynh Giang Le,Yukio Kimata
Quynh Giang Le
Dysfunction of the endoplasmic reticulum (ER), so-called ER stress, is accompanied with accumulation of unfolded proteins in the ER. Eukaryotic cells commonly have an ER-located transmembrane protein, Ire1, which triggers cellular protectiv...
Knock-in of Labeled Proteins into 5'UTR Enables Highly Efficient Generation of Stable Cell Lines [0.03%]
5'-UTR定位报告蛋白高效稳定细胞系的产生技术knock-in技术
Faryal Ijaz,Koji Ikegami
Faryal Ijaz
Stable cell lines and animal models expressing tagged proteins are important tools for studying behaviors of cells and molecules. Several molecular biology technologies have been applied with varying degrees of success and efficiencies to e...
Dispensability of Tubulin Acetylation for 15-protofilament Microtubule Formation in the Mammalian Cochlea [0.03%]
哺乳动物耳蜗中15个原纤维蛋白丝微管形成的泛素乙酰化可有可无
Justine Renauld,Nicolas Thelen,Odile Bartholomé et al.
Justine Renauld et al.
The development of hearing in mammals requires the formation and maturation of a highly organized and specialized epithelium known as the organ of Corti. This epithelium contains two types of cells, the sensory cells, which are the true rec...
Mevalonate Pathway-mediated ER Homeostasis Is Required for Haploid Stability in Human Somatic Cells [0.03%]
人胚肺细胞中异柠檬酸脱氢酶突变通过甲羟戊酸途径导致内质网应激及单倍体稳定性丧失
Kan Yaguchi,Kimino Sato,Koya Yoshizawa et al.
Kan Yaguchi et al.
The somatic haploidy is unstable in diplontic animals, but cellular processes determining haploid stability remain elusive. Here, we found that inhibition of mevalonate pathway by pitavastatin, a widely used cholesterol-lowering drug, drast...
An Antiviral Drug Screening Platform with a FRET Biosensor for Measurement of Arenavirus Z Assembly [0.03%]
一种通过FRET生物传感器测量雅尔塔病毒Z组装进行抗病毒药物筛选的平台
Tatsuaki Mizutani,Yusuke Ohba,Satoshi Mizuta et al.
Tatsuaki Mizutani et al.
The smallest arenavirus gene product, Z protein, plays critical roles in the virus life cycle. Z is the major driving force of budding and particle production because of a unique property that defines self-assembly. In addition to the roles...
Prickle2 and Igsf9b Coordinately Regulate the Cytoarchitecture of the Axon Initial Segment [0.03%]
刺细胞和Igsf9b协调调节轴突初始段的细胞架构
Md Imrul Hasan Chowdhury,Tomoki Nishioka,Noriko Mishima et al.
Md Imrul Hasan Chowdhury et al.
Prickle2 has been identified in genetic studies of subjects with autism spectrum disorder (ASD) and epilepsy, but the pathological mechanism of Prickle2 remains to be fully understood. Proteomic analysis of Prickle2 with mass spectrometry r...