Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells [0.03%]
在上皮细胞中用单独的Claudin亚型重构功能性紧密连接
Mikio Furuse,Daiki Nakatsu,Wendy Hempstock et al.
Mikio Furuse et al.
The claudin family of membrane proteins is responsible for the backbone structure and function of tight junctions (TJs), which regulate the paracellular permeability of epithelia. It is thought that each claudin subtype has its own unique f...
Quantitative 3D correlative light and electron microscopy of organelle association during autophagy [0.03%]
自噬过程中细胞器关联的定量三维光电子相关显微镜观察
Satoru Takahashi,Chieko Saito,Ikuko Koyama-Honda et al.
Satoru Takahashi et al.
In macroautophagy, disk-shaped double-membrane structures called phagophores elongate to form cup-shaped structures, becoming autophagosomes upon closure. These autophagosomes then fuse with lysosomes to become autolysosomes and degrade eng...
PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis [0.03%]
PRL通过在有丝分裂期间抑制着丝粒定位的AMPK活化来刺激有丝分裂错误
Kajung Ryu,Atsushi Yoshida,Yosuke Funato et al.
Kajung Ryu et al.
Phosphatase of regenerating liver (PRL) is frequently overexpressed in various malignant cancers and is known to be a driver of malignancy. Here, we demonstrated that PRL overexpression causes mitotic errors that accompany spindle misorient...
Appropriate tension sensitivity of α-catenin ensures rounding morphogenesis of epithelial spheroids [0.03%]
α-E连环蛋白合适的张力敏感性确保上皮球状体圆形化形态发生
Ryosuke Nishimura,Kagayaki Kato,Misako Saida et al.
Ryosuke Nishimura et al.
The adherens junction (AJ) is an actin filament-anchoring junction. It plays a central role in epithelial morphogenesis through cadherin-based recognition and adhesion among cells. The stability and plasticity of AJs are required for the mo...
A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields [0.03%]
一种产生高产量的SARS冠状病毒刺突蛋白伪病毒颗粒的方法
Yoichiro Fujioka,Sayaka Kashiwagi,Aiko Yoshida et al.
Yoichiro Fujioka et al.
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has threatened human health and the global economy. Development of additional vaccines and therapeutics is urgently required, but such development with live v...
Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion [0.03%]
Munc13b在融合前受刺激调控积累到Granuphilin介导的docked囊泡上
Kouichi Mizuno,Tetsuro Izumi
Kouichi Mizuno
The Rab27 effector granuphilin plays an indispensable role in stable docking of secretory granules to the plasma membrane by interacting with the complex of Munc18-1 and the fusion-incompetent, closed form of syntaxins-1~3. Although this pr...
Ubiquitin-like 3 as a new protein-sorting factor for small extracellular vesicles [0.03%]
泛素类似物3是小细胞外囊泡的新的蛋白质分类因子
Yusuke Takanashi,Tomoaki Kahyo,Sae Kamamoto et al.
Yusuke Takanashi et al.
Ubiquitin-like 3 (UBL3) is a well-conserved ubiquitin-like protein (UBL) in eukaryotes and regulates the ubiquitin cascade, but the significant roles of UBL3 in cellular processes remained unknown. Recently, UBL3 was elucidated to be a post...
Specific association of TBK1 with the trans-Golgi network following STING stimulation [0.03%]
TBK1在STING刺激后的反式高尔基网络的特异性结合
Haruka Kemmoku,Yoshihiko Kuchitsu,Kojiro Mukai et al.
Haruka Kemmoku et al.
Stimulator of interferon genes (STING) is essential for the type I interferon response induced by microbial DNA or self-DNA leaked from mitochondria/nuclei. In response to the emergence of such DNAs in the cytosol, STING relocates from the ...
A Dual Promoter System to Monitor IFN-γ Signaling in vivo at Single-cell Resolution [0.03%]
用于在活体中以单细胞分辨率监测IFN-γ信号的双启动子系统
Taisei Tanaka,Yoshinobu Konishi,Hiroshi Ichise et al.
Taisei Tanaka et al.
IFN-γ secreted from immune cells exerts pleiotropic effects on tumor cells, including induction of immune checkpoint and antigen presentation, growth inhibition, and apoptosis induction. We combined a dual promoter system with an IFN-γ si...
Visualization of DNA Replication in Single Chromosome by Stable Isotope Labeling [0.03%]
稳定同位素标记单染色体DNA复制的可视化研究
Kosuke Nagata,Ken-Ichi Bajo,Hideyuki Mitomo et al.
Kosuke Nagata et al.
Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with...