Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression [0.03%]
两种不同颜色的珊瑚荧光蛋白可清晰地观察细胞周期进程
Ryoko Ando,Asako Sakaue-Sawano,Keiko Shoda et al.
Ryoko Ando et al.
We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red...
Proteomic analysis of fatty liver induced by starvation of medaka fish larvae [0.03%]
斑马鱼饥饿诱导脂肪肝的蛋白质组分析
Tomoyo Ikeda,Tokiro Ishikawa,Satoshi Ninagawa et al.
Tomoyo Ikeda et al.
When medaka fish (Oryzias latipes) larvae are grown in the absence of exogenous nutrition, the liver becomes dark and positive to Oil Red O staining from 7 days post-hatch (dph). We determined the mechanism of this starvation-induced develo...
Zebrafish imaging reveals hidden oncogenic-normal cell communication during primary tumorigenesis [0.03%]
斑马鱼成像揭示原发肿瘤发生过程中隐藏的致癌细胞与正常细胞之间的通讯现象
Yukinari Haraoka,Mai Miyake,Tohru Ishitani
Yukinari Haraoka
Oncogenic mutations drive tumorigenesis, and single cells with oncogenic mutations act as the tumor seeds that gradually evolve into fully transformed tumors. However, oncogenic cell behavior and communication with neighboring cells during ...
Ken&#x;ichiro Hayashi,Shinri Horoiwa,Kotaro Mori et al.
Ken&#x;ichiro Hayashi et al.
Inflammatory response induces phenotypic modulation of fibroblasts into myofibroblasts. Although transforming growth factor-βs (TGF-βs) evoke such transition, the details of the mechanism are still unknown. Here, we report that a LIM doma...
Jun-Ichi Sakamaki,Noboru Mizushima
Jun-Ichi Sakamaki
Protein-lipid conjugation is a widespread modification involved in many biological processes. Various lipids, including fatty acids, isoprenoids, sterols, glycosylphosphatidylinositol, sphingolipids, and phospholipids, are covalently linked...
A conserved WXXE motif is an apical delivery determinant of ABC transporter C subfamily isoforms [0.03%]
保守的WXXE基序是ABC转运蛋白C亚家族同工型向顶运送决定因子
Md Shajedul Haque,Yoshikazu Emi,Masao Sakaguchi
Md Shajedul Haque
ATP-binding cassette transporter isoform C7 (ABCC7), also designated as cystic fibrosis transmembrane conductance regulator (CFTR), is exclusively targeted to the apical plasma membrane of polarized epithelial cells. Although the apical loc...
A non-nucleotide agonist that binds covalently to cysteine residues of STING [0.03%]
与STING的半胱氨酸残基共价结合的非核苷酸激动剂
Kentaro Matsumoto,Shenwei Ni,Hiroyuki Arai et al.
Kentaro Matsumoto et al.
Stimulator of interferon genes (STING) is an ER-localized transmembrane protein and the receptor for 2',3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP), which is a second messenger produced by cGAMP synthase (cGAS), a cyto...
Suppression of P-cadherin expression as a key regulatory element for embryonic stem cell stemness [0.03%]
P-钙粘着蛋白表达的抑制作为胚胎干细胞干性的关键调控元件
Yuka Takeda,Shuji Matsuguchi,Sae Nozaki et al.
Yuka Takeda et al.
In embryonic stem (ES) cell colonies, a small subpopulation that changes cell shape and loses pluripotency often appears in two-dimensional (2D) cultures, even in the presence of a stemness factor. We have previously shown that membrane tra...
Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells [0.03%]
基于Vivid的光活化Gal4转录因子在哺乳动物细胞中的增强效应
Shinji C Nagasaki,Tomonori D Fukuda,Mayumi Yamada et al.
Shinji C Nagasaki et al.
The Gal4/UAS system is a versatile tool to manipulate exogenous gene expression of cells spatially and temporally in many model organisms. Many variations of light-controllable Gal4/UAS system are now available, following the development of...
Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast [0.03%]
磷脂酰丝氨酸在酵母细胞表面膜蛋白定位中的作用
Ryutaro Kashikuma,Makoto Nagano,Hiroki Shimamura et al.
Ryutaro Kashikuma et al.
Phosphatidylserine (PS) is a constituent of the cell membrane, being especially abundant in the cytoplasmic leaflet, and plays important roles in a number of cellular functions, including the formation of cell polarity and intracellular ves...