Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice [0.03%]
肝特异性ZO-1和ZO-2基因敲除小鼠相关病理生理的分子改变
Masahiko Itoh,Kenji Watanabe,Yoichi Mizukami et al.
Masahiko Itoh et al.
The liver is a complex organ with a highly organized structure in which tight junctions (TJs) play an important role in maintaining their function by regulating barrier properties and cellular polarity. Dysfunction of TJs is associated with...
Yuan Ximin,Hitoshi Hashimoto,Ikuo Wada et al.
Yuan Ximin et al.
Collagen is the most abundant protein in the extracellular matrix of animals, and 28 types of collagen have been reported in humans. We previously analyzed the endoplasmic reticulum (ER)-to-Golgi transport of fibril-forming type III collage...
Deep learning-based segmentation of subcellular organelles in high-resolution phase-contrast images [0.03%]
基于深度学习的高分辨率相位对比图像中细胞器分割
Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito et al.
Kentaro Shimasaki et al.
Although quantitative analysis of biological images demands precise extraction of specific organelles or cells, it remains challenging in broad-field grayscale images, where traditional thresholding methods have been hampered due to complex...
Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging [0.03%]
利用三维超分辨率成像解析糖基转移酶的次级高尔基体定位机制
Hirokazu Yagi,Seigo Tateo,Taiki Saito et al.
Hirokazu Yagi et al.
The Golgi apparatus, a crucial organelle involved in protein processing, including glycosylation, exhibits complex sub-structures, i.e., cis-, medial, and trans-cisternae. This study investigated the distribution of glycosyltransferases wit...
Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells [0.03%]
半完完整细胞中染色体的有丝分裂后核膜亚域重构
Tomoko Funakoshi,Naoko Imamoto
Tomoko Funakoshi
In metazoans, the nuclear envelope (NE) disassembles during the prophase and reassembles around segregated chromatids during the telophase. The process of NE formation has been extensively studied using live-cell imaging. At the early step ...
A high-resolution phase-contrast microscopy system for label-free imaging in living cells [0.03%]
一种用于活细胞中无标记成像的高分辨率相位对比显微镜系统
Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito et al.
Kentaro Shimasaki et al.
Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better vi...
In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila [0.03%]
果蝇中核糖体蛋白水平同态调节的体内证据
Daiki Kitamura,Kiichiro Taniguchi,Mai Nakamura et al.
Daiki Kitamura et al.
The ribosome is a molecular machine essential for protein synthesis, which is composed of approximately 80 different ribosomal proteins (Rps). Studies in yeast and cell culture systems have revealed that the intracellular level of Rps is fi...
Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease [0.03%]
Gaucher病患者来源的成纤维细胞溶酶体膜完整性的改变
Asuka Hamamoto,Natsuki Kita,Siddabasave Gowda B Gowda et al.
Asuka Hamamoto et al.
Gaucher disease (GD) is a recessively inherited lysosomal storage disorder characterized by a deficiency of lysosomal glucocerebrosidase (GBA1). This deficiency results in the accumulation of its substrate, glucosylceramide (GlcCer), within...
Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium [0.03%]
冠蛋白1通过促进斑翅果蝇表皮细胞的极性重排来抑制细菌感染引起的过度炎症反应
Keisuke Ikawa,Souta Hiro,Shu Kondo et al.
Keisuke Ikawa et al.
Directional cell rearrangement is a critical process underlying correct tissue deformation during morphogenesis. Although the involvement of F-actin regulation in cell rearrangement has been established, the role and regulation of actin bin...
Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition [0.03%]
p38MAPK-CRP2轴在肌成纤维细胞表型转变中的意义
Ken&#x;ichiro Hayashi,Reuben Jacob Labios,Tsuyoshi Morita et al.
Ken&#x;ichiro Hayashi et al.
We have recently demonstrated that a LIM domain protein, cysteine and glycine-rich protein 2 (CSRP2 [CRP2]), plays a vital role in the functional expression of myofibroblasts and cancer-associated fibroblasts. CRP2 binds directly to myocard...