Possible roles of CAHS proteins from Tardigrade in osmotic stress tolerance in mammalian cells [0.03%]
缓步动物CAHS蛋白可能在哺乳动物细胞渗透胁迫耐受中扮演的作用
Takahiro Bino,Yuhei Goto,Gembu Maryu et al.
Takahiro Bino et al.
Anhydrobiosis, a phenomenon in which organisms survive extreme dehydration by entering a reversible ametabolic state, is a remarkable example of survival strategies. This study focuses on anhydrobiosis in tardigrades, which are known for th...
The expression of the formin Fhod3 in mouse tongue striated muscle [0.03%]
小鼠舌横纹肌formin家族成员Fhod3的表达式异变研究
Hikaru Nakagawa,Yohko Kage,Ayako Miura et al.
Hikaru Nakagawa et al.
The sarcomere is the contractile unit of striated muscle and is composed of actin and myosin filaments. There is increasing evidence to support that actin assembly mediated by Fhod3, a member of the formin family of proteins, is critical fo...
Cell-wide arrangement of Golgi/RE units depends on the microtubule organization [0.03%]
细胞内高尔基体/内质网单位的排列取决于微管的组织方式
Tatsuya Tago,Syara Fujii,Shogo Sasaki et al.
Tatsuya Tago et al.
We have previously shown that Golgi stacks and recycling endosomes (REs) exist as Golgi/RE units in sea urchin embryos. In this study, we showed that Golgi/RE units were scattered throughout the cytoplasm at early developmental stages but g...
Molecular alterations associated with pathophysiology in liver-specific ZO-1 and ZO-2 knockout mice [0.03%]
肝特异性ZO-1和ZO-2基因敲除小鼠相关病理生理的分子改变
Masahiko Itoh,Kenji Watanabe,Yoichi Mizukami et al.
Masahiko Itoh et al.
The liver is a complex organ with a highly organized structure in which tight junctions (TJs) play an important role in maintaining their function by regulating barrier properties and cellular polarity. Dysfunction of TJs is associated with...
Yuan Ximin,Hitoshi Hashimoto,Ikuo Wada et al.
Yuan Ximin et al.
Collagen is the most abundant protein in the extracellular matrix of animals, and 28 types of collagen have been reported in humans. We previously analyzed the endoplasmic reticulum (ER)-to-Golgi transport of fibril-forming type III collage...
Deep learning-based segmentation of subcellular organelles in high-resolution phase-contrast images [0.03%]
基于深度学习的高分辨率相位对比图像中细胞器分割
Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito et al.
Kentaro Shimasaki et al.
Although quantitative analysis of biological images demands precise extraction of specific organelles or cells, it remains challenging in broad-field grayscale images, where traditional thresholding methods have been hampered due to complex...
Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging [0.03%]
利用三维超分辨率成像解析糖基转移酶的次级高尔基体定位机制
Hirokazu Yagi,Seigo Tateo,Taiki Saito et al.
Hirokazu Yagi et al.
The Golgi apparatus, a crucial organelle involved in protein processing, including glycosylation, exhibits complex sub-structures, i.e., cis-, medial, and trans-cisternae. This study investigated the distribution of glycosyltransferases wit...
Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells [0.03%]
半完完整细胞中染色体的有丝分裂后核膜亚域重构
Tomoko Funakoshi,Naoko Imamoto
Tomoko Funakoshi
In metazoans, the nuclear envelope (NE) disassembles during the prophase and reassembles around segregated chromatids during the telophase. The process of NE formation has been extensively studied using live-cell imaging. At the early step ...
A high-resolution phase-contrast microscopy system for label-free imaging in living cells [0.03%]
一种用于活细胞中无标记成像的高分辨率相位对比显微镜系统
Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito et al.
Kentaro Shimasaki et al.
Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better vi...
In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila [0.03%]
果蝇中核糖体蛋白水平同态调节的体内证据
Daiki Kitamura,Kiichiro Taniguchi,Mai Nakamura et al.
Daiki Kitamura et al.
The ribosome is a molecular machine essential for protein synthesis, which is composed of approximately 80 different ribosomal proteins (Rps). Studies in yeast and cell culture systems have revealed that the intracellular level of Rps is fi...