Mature adipocyte-derived dedifferentiated fat cells can trans-differentiate into osteoblasts in vitro and in vivo only by all-trans retinoic acid [0.03%]
成熟脂肪细胞衍生的去分化脂肪细胞只能在全反式视黄酸作用下向成骨细胞转化
Yoshinao Oki,Saiko Watanabe,Tuyoshi Endo et al.
Yoshinao Oki et al.
We investigated whether de-differentiated fat (DFAT) cells, a mature adipocyte-derived preadipocyte cell line, can be induced to trans-differentiate into osteoblasts in vitro and in vivo. All-trans retinoic acid (RA) induced expression of o...
Mechanical strain increases expression of type XII collagen in murine osteoblastic MC3T3-E1 cells [0.03%]
机械牵张应变可诱导小鼠成骨细胞系MC3T3-E1中XII型胶原基因表达量增加
Katsuhiko Arai,Yuko Nagashima,Taeko Takemoto et al.
Katsuhiko Arai et al.
In adult mouse, the mRNA corresponding to the alpha1 chain of type XII collagen (alpha 1(XII)) is predominantly detected in the bone. Additionally, murine osteoblastic cells, MC3T3-E1, increased the mRNA level of alpha 1(XII) response to th...
Suppression of cardiac troponin T induces reduction of contractility and structural disorganization in chicken cardiomyocytes [0.03%]
卡托普利n抑制鸡心肌细胞心肌肌钙蛋白T的表达降低心肌收缩力和引起肌原纤维排列紊乱
Naoji Toyota,Hiromi Takano-Ohmuro,Lucia S Yoshida et al.
Naoji Toyota et al.
We herein examine the effect of cardiac troponin T (CTnT) suppression in cultured chicken cardiomyocytes derived from embryonic cardiac ventricular muscle. TnT is an important protein participating in regulation of striated muscle contracti...
Cross-talk-free fluorescence cross-correlation spectroscopy by the switching method [0.03%]
开关法无串扰的荧光交联相关光谱法
Yasuo Takahashi,Junichi Nishimura,Akemi Suzuki et al.
Yasuo Takahashi et al.
Fluorescence cross-correlation spectroscopy (FCCS) is used as a powerful technique to analyze molecular interactions both in vitro and in vivo. This method basically requires two laser excitations for two target molecules labeled with fluor...
MicroRNA-206 is highly expressed in newly formed muscle fibers: implications regarding potential for muscle regeneration and maturation in muscular dystrophy [0.03%]
微小核糖核酸-206在新生肌纤维中高表达及其对肌肉再生和营养不良的影响
Katsutoshi Yuasa,Yasuko Hagiwara,Masanori Ando et al.
Katsutoshi Yuasa et al.
miR-1, miR-133a, and miR-206 are muscle-specific microRNAs expressed in skeletal muscles and have been shown to contribute to muscle development. To gain insight into the pathophysiological roles of these three microRNAs in dystrophin-defic...
SYP71, a plant-specific Qc-SNARE protein, reveals dual localization to the plasma membrane and the endoplasmic reticulum in Arabidopsis [0.03%]
植物特有的Qc-SNARE蛋白SYP71在拟南芥中定位于质膜和内质网二者兼有
I Nengah Suwastika,Tomohiro Uemura,Takashi Shiina et al.
I Nengah Suwastika et al.
SNAREs ('Soluble N-ethyl-maleimide sensitive factor attachment protein receptors') play a critical role in the membrane fusion step of the vesicular transport system in eukaryotes. The number of the genes encoding SNARE proteins is estimate...
Migratory localization of cyclin D2-Cdk4 complex suggests a spatial regulation of the G1-S transition [0.03%]
cyclin D2-Cdk4复合物的迁徙定位暗示了G1-S转换的空间调节
Zizhang Wang,Yongli Xie,Lixia Zhang et al.
Zizhang Wang et al.
The association of the cyclin D-Cdk (DC) complex with retinoblastoma protein (pRb) is required for the G1-S transition of the cell cycle. Cyclin synthesis, nuclear localization and degradation are control mechanisms for the transition, but ...
Involvement of a 25 kDa Tetrahymena Ca2+-binding protein in pronuclear exchange [0.03%]
一个25kD的Tetrahymena钙结合蛋白参与核交换反应
Tomomi Nakagawa,Kenta Fujiu,Eric S Cole et al.
Tomomi Nakagawa et al.
The Tetrahymena Ca2+-binding protein of 25 kDa (TCBP-25) is a calmodulin family protein containing four EF-hand type calcium-binding domains. TCBP-25 is localized in the whole cell cortex and around both the migratory and stationary pronucl...
A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function [0.03%]
一种基于汞弧灯的多色共聚焦实时成像系统用于细胞结构与功能研究
Kenta Saito,Kentaro Kobayashi,Tomomi Tani et al.
Kenta Saito et al.
Multi-point scanning confocal microscopy using a Nipkow disk enables the acquisition of fluorescent images with high spatial and temporal resolutions. Like other single-point scanning confocal systems that use Galvano meter mirrors, a comme...
Ultrastructural study of Rac1 and its effectors beneath the substratum-facing membrane [0.03%]
Rac1及其效应物在胞底膜下的超微结构研究
Takashi Watanabe,Shujie Wang,Mai Kakeno et al.
Takashi Watanabe et al.
The cytoskeletal architecture and adhesion apparatus are tightly controlled during embryogenesis, tissue development, and carcinogenesis. The Rho family GTPases play central roles in regulation of the cytoskeleton and adhesions. Rac1, one o...