Letizia Venturini,Matthias Eder,Michaela Scherr
Letizia Venturini
In the past few years, the discovery of RNA-mediated gene silencing mechanisms, like RNA interference (RNAi), has revolutionized our understanding of eukaryotic gene expression. These mechanisms are activated by double-stranded RNA (dsRNA) ...
Jens Kurreck
Jens Kurreck
The triumphant success of RNA interference (RNAi) in life sciences is based on its high potency to silence genes in a sequence-specific manner. Nevertheless, the first task for successful RNAi approaches is the identification of highly acti...
Laura Cerchia,Vittorio De Franciscis
Laura Cerchia
Several signalling proteins involved in cell growth and differentiation represent attractive candidate targets for cancer diagnosis and/or therapy since they can act as oncogenes. Because of their high specificity and low immunogeneicity, u...
Delivery systems for the direct application of siRNAs to induce RNA interference (RNAi) in vivo [0.03%]
用于在活体内直接应用siRNA诱导RNA干扰的递送系统
Achim Aigner
Achim Aigner
RNA interference (RNAi) is a powerful method for specific gene silencing which may also lead to promising novel therapeutic strategies. It is mediated through small interfering RNAs (siRNAs) which sequence-specifically trigger the cleavage ...
Dominique L Ouellet,Marjorie P Perron,Lise-Andrée Gobeil et al.
Dominique L Ouellet et al.
Encoded by the genome of most eukaryotes examined so far, microRNAs (miRNAs) are small approximately 21-nucleotide (nt) noncoding RNAs (ncRNAs) derived from a biosynthetic cascade involving sequential processing steps executed by the ribonu...
Essential notes regarding the design of functional siRNAs for efficient mammalian RNAi [0.03%]
关于功能性siRNA设计以提高哺乳动物RNA干扰效率的必要注意事项
Kumiko Ui-Tei,Yuki Naito,Kaoru Saigo
Kumiko Ui-Tei
Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. C...
Dicer-derived microRNAs are utilized by the fragile X mental retardation protein for assembly on target RNAs [0.03%]
Dicer衍生的microRNA被脆性X染色体综合征智力迟滞蛋白用于靶标RNA组装
Isabelle Plante,Laetitia Davidovic,Dominique L Ouellet et al.
Isabelle Plante et al.
In mammalian cells, fragile X mental retardation protein (FMRP) has been reported to be part of a microRNA (miRNA)-containing effector ribonucleoprotien (RNP) complex believed to mediate translational control of specific mRNAs. Here, using ...
Targeted delivery of siRNA [0.03%]
靶向递送siRNA
Sabrina Oliveira,Gert Storm,Raymond M Schiffelers
Sabrina Oliveira
Therapeutic application of siRNA requires delivery to the correct intracellular location, to interact with the RNAi machinery within the target cell, within the target tissue responsible for the pathology. Each of these levels of targeting ...
Julie Clark,Sheng Ding
Julie Clark
The completion of the genome sequencing for several organisms has created a great demand for genomic tools that can systematically analyze the growing wealth of data. In contrast to the classical reverse genetics approach of creating specif...
Adenovirus-mediated in vivo silencing of anaphylatoxin receptor C5aR [0.03%]
腺病毒介导的过敏毒素受体C5aR的体内沉默作用
Lei Sun,Hongwei Gao,Vidya J Sarma et al.
Lei Sun et al.
C5a, one of the most potent inflammatory peptides, induces its inflammatory functions by interacting with C5a receptor (C5aR) that belongs to the rhodopsin family of seven-transmembrane G protein-coupled receptors. C5a/C5aR signaling has be...