Pilot-Scale Production of Peroxygenase from Agrocybe aegerita [0.03%]
一株 Agrocybe aegerita 过氧化酶的升尺度生产
Fabio Tonin,Florian Tieves,Sébastien Willot et al.
Fabio Tonin et al.
The pilot-scale production of the peroxygenase from Agrocybe aegerita (rAaeUPO) is demonstrated. In a fed-batch fermentation of the recombinant Pichia pastoris, the enzyme was secreted into the culture medium to a final concentration of 0.2...
Igor V Kolesnichenko,Galina Z Goloverda,Vladimir L Kolesnichenko
Igor V Kolesnichenko
Isolation of heat-sensitive reaction products in post-synthesis workup procedures often requires ambient-or low-temperature solvent removal. In the method demonstrated here, solvent evaporation is driven by the pressure gradient between a d...
Zohaib R Siddiqi,Chad N Ungarean,Tanner W Bingham et al.
Zohaib R Siddiqi et al.
The cyclic azodicarbonyl 4-methyl-1,2,4-triazoline-3,5-dione (MTAD) is a versatile and powerful reagent used mainly in cycloaddition chemistry. Though known for more than 50 years, its unsafe preparation, as well as purification by sublimat...
Exploring the Role of Anti-solvent Effects during Washing on Active Pharmaceutical Ingredient Purity [0.03%]
探讨洗涤过程中反溶剂效应对于活性药物成分纯度的影响
Muhid Shahid,Georgia Sanxaridou,Sara Ottoboni et al.
Muhid Shahid et al.
Washing is a key step in pharmaceutical isolation to remove the unwanted crystallization solvent (mother liquor) from the active pharmaceutical ingredient (API) filter cake. This study looks at strategies for optimal wash solvent selection,...
Rafal M Dziedzic,Jonathan C Axtell,Arnold L Rheingold et al.
Rafal M Dziedzic et al.
Off-cycle processes in catalytic reactions can dramatically influence the outcome of the chemical transformation and affect its yield, selectivity, rate, and product distribution. While the generation of off-cycle intermediates can complica...
Toward the Scale-Up of a Bicyclic Homopiperazine via Schmidt Rearrangement and Photochemical Oxaziridine Rearrangement in Continuous-Flow [0.03%]
通过施密特重排和连续流动中的光化学氧氮六环重排实现 bicyclic homopiperazine 规模扩大化的研究
Michael Brown,Mohammed Aljarah,Hannah Asiki et al.
Michael Brown et al.
The scale-up of a chiral bicyclic homopiperazine of pharmaceutical interest was investigated. The outcome and safety profile of a key batch ring-expansion step via Schmidt rearrangement was improved using continuous-flow chemistry. The sele...
Pd-Catalyzed Alkene Difunctionalization Reactions of Enolates for the Synthesis of Substituted Bicyclic Cyclopentanes [0.03%]
钯催化烯烃与烯醇化物的双官能化反应合成取代螺环环戊烷化合物
Evan C Bornowski,Elsa M Hinds,Derick R White et al.
Evan C Bornowski et al.
Palladium-catalyzed alkene difunctionalization reactions between alkenes bearing tethered aryl or alkenyl triflates and enolate nucleophiles are described. The transformations form two C-C bonds, a ring, and up to two stereocenters, while p...
Understanding API Static Drying with Hot Gas Flow: Design and Test of a Drying Rig Prototype and Drying Modeling Development [0.03%]
基于热风干燥的API静态干燥试验装置设计及干燥模型研究
Sara Ottoboni,Simon J Coleman,Christopher Steven et al.
Sara Ottoboni et al.
Developing a continuous isolation process to produce a pure, dry, free-flowing active pharmaceutical ingredient (API) is the final barrier to the implementation of continuous end-to-end pharmaceutical manufacturing. Recent work has led to t...
Optimization of a Decatungstate-Catalyzed C(sp3)-H Alkylation Using a Continuous Oscillatory Millistructured Photoreactor [0.03%]
连续振荡微结构光反应器对钨氧簇催化C(sp3)-H烷基化反应的优化
Zhenghui Wen,Apoorva Maheshwari,Carlo Sambiagio et al.
Zhenghui Wen et al.
Tetrabutylammonium decatungstate (TBADT) has emerged as an efficient and versatile photocatalyst for hydrogen atom transfer (HAT) processes that enables the cleavage of both activated and unactivated aliphatic C-H bonds. Using a recently de...
Barnabas Poznansky,Lisa A Thompson,Sarah A Warren et al.
Barnabas Poznansky et al.
A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-imm...