Alexey A Pakhomov,Vladimir I Martynov
Alexey A Pakhomov
Proteins homologous to green fluorescent protein (GFP) span most of the visible spectrum, offering indispensable tools for live cell imaging. Structural transformations, such as posttranslational autocatalytic and photo-induced modification...
Terry T Takahashi,Shuwei Li
Terry T Takahashi
MacBeath and colleagues (Kaushansky et al., 2008) use a protein array technology to find binding partners of ErbB4 in a genome-wide and quantitative fashion, shedding new light on how ErbB4 initiates cellular signaling events and why ErbB4 ...
Wendy Wolfson
Wendy Wolfson
Glycosidase inhibition by macrolide antibiotics elucidated by STD-NMR spectroscopy [0.03%]
利用STD-NMR光谱法阐明大环内酯类抗生素抑制糖基化酶的机制
Ali Sadeghi-Khomami,Michael D Lumsden,David L Jakeman
Ali Sadeghi-Khomami
A glycosynthase approach was attempted to glycodiversify macrolide antibiotics, using DesR, a family-3 retaining beta-glucosidase involved in the self-resistance mechanism of methymycin production. STD-NMR was used to probe enzyme-substrate...
Tryptophan aminopeptidase activity of several indole prenyltransferases from Aspergillus fumigatus [0.03%]
雾枝曲霉几种吲哚牻牛儿基转移酶的色氨酸氨基肽酶活性
Anika Kremer,Shu-Ming Li
Anika Kremer
Recently, five indole prenyltransferases from Aspergillus fumigatus have been proven biochemically to be responsible for prenylations of diverse substrates. In this study, we show peptidase activities of 7-DMATS, FgaPT1, CdpNPT, and FtmPT1,...
Nanomolar CFTR inhibition by pore-occluding divalent polyethylene glycol-malonic acid hydrazides [0.03%]
纳摩尔浓度的CFTR抑制剂通过二价聚乙二醇丁二酸肼封闭通道实现
N D Sonawane,Dan Zhao,Olga Zegarra-Moran et al.
N D Sonawane et al.
Inhibitors of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel have potential application as antisecretory therapy in cholera. We synthesized mono- and divalent CFTR inhibitors consisting of a malonic acid hyd...
Bacterial base excision repair enzyme Fpg recognizes bulky N7-substituted-FapydG lesion via unproductive binding mode [0.03%]
细菌碱基切除修复酶Fpg通过非产物形成结合模式识别大体积的N7取代型-Fapy-G lesions
Franck Coste,Matthias Ober,Yann-Vaï Le Bihan et al.
Franck Coste et al.
Fpg is a bacterial base excision repair enzyme that removes oxidized purines from DNA. This work shows that Fpg and its eukaryote homolog Ogg1 recognize with high affinity FapydG and bulky N7-benzyl-FapydG (Bz-FapydG). The comparative cryst...
Characterization of the azinomycin B biosynthetic gene cluster revealing a different iterative type I polyketide synthase for naphthoate biosynthesis [0.03%]
B噬菌体整合位点旁侧序列中抗性基因的获得和丢失机制研究
Qunfei Zhao,Qingli He,Wei Ding et al.
Qunfei Zhao et al.
Azinomycin B is a complex natural product containing densely assembled functionalities with potent antitumor activity. Cloning and sequence analysis of the azi gene cluster revealed an iterative type I polyketide synthase (PKS) gene, five n...
Identification of polyoxometalates as nanomolar noncompetitive inhibitors of protein kinase CK2 [0.03%]
鉴定出多金属氧酸盐作为蛋白激酶CK2的纳摩尔非竞争性抑制剂
Renaud Prudent,Virginie Moucadel,Béatrice Laudet et al.
Renaud Prudent et al.
Protein kinase CK2 is a multifunctional kinase of medical importance that is dysregulated in many cancers. In this study, polyoxometalates were identified as original CK2 inhibitors. [P2Mo18O62](6-) has the most potent activity. It inhibits...
Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer [0.03%]
在分子支架上组装OX40适配体以创建激活受体的适配体
Claudia M Dollins,Smita Nair,David Boczkowski et al.
Claudia M Dollins et al.
We show that a molecular scaffold can be utilized to convert a receptor binding aptamer into a receptor agonist. Many receptors (including tumor necrosis receptor family members) are activated when they are multimerized on the cell surface....