Production of transgenic cashmere goat embryos expressing red fluorescent protein and containing IGF1 hair-follicle-cell specific expression cassette by somatic cell nuclear transfer [0.03%]
体细胞克隆技术生产含IGF1基因的荧光山羊胚胎
XuDong Guo,DongShan Yang,XuDong Ao et al.
XuDong Guo et al.
In the present study, cashmere goat fetal fibroblasts were transfected with pCDsR-KI, a hair-follicle-cell specific expression vector for insulin-like growth factor 1 (IGF1) that contains two markers for selection (red fluorescent protein g...
Arginine bi-directional translocation and breakdown into ornithine along the arbuscular mycorrhizal mycelium [0.03%]
无隔菌丝双向转运精氨素并将其分解为鸟氨酸
HaiRu Jin
HaiRu Jin
Bi-directional translocation and degradation of Arginine (Arg) along the arbuscular mycorrhizal (AM) fungal mycelium were testified through (15)N and/or (13)C isotopic labeling. In vitro mycorrhizas of Glomus intraradices and Ri T-DNA-trans...
Protein identification and mRNA analysis of phytochrome-regulated genes in Arabidopsis under red light [0.03%]
红光下阿拉伯芥中phytochrome调节基因的蛋白质鉴定及mRNA分析
Xu Li,YueJun Yang,Yan Li et al.
Xu Li et al.
Phytochromes are a family of plant photoreceptors that mediate physiological and developmental responses to red and far-red light. According to the affymetrix ATH1 microarray, phytochrome A (phyA) and phytochrome B (phyB) together play a ke...
Endothelin-1 induces intracellular [Ca2+] increase via Ca 2+ influx through the L-type Ca2+ channel, Ca 2+ -induced Ca2+ release and a pathway involving ET A receptors, PKC, PKA and AT1 receptors in cardiomyocytes [0.03%]
内皮素-1通过L型钙通道Ca2+内流、Ca2+-诱导的Ca2+释放以及ETa受体、PKC、PKA和AT1受体相关信号途径在心肌细胞中诱导胞浆游离Ca2+浓度升高
QingHua Zeng,XingTing Li,GuoGan Zhong et al.
QingHua Zeng et al.
Using fura-2-acetoxymethyl ester (AM) fluorescence imaging and patch clamp techniques, we found that endothelin-1 (ET-1) significantly elevated the intracellular calcium level ([Ca(2+)](i)) in a dose-dependent manner and activated the L-typ...
ShuLan Qiu,JiaNing Wang,Chuang Yu et al.
ShuLan Qiu et al.
Kinetochores are large proteinaceous structure on the surface of chromosomes' primary constriction during mitosis. They link chromosomes to spindle microtubules and also regulate the spindle assembly checkpoint, which is crucial for correct...
Transcriptional regulation of the Herpes Simplex Virus 1alpha-gene by the viral immediate-early protein ICP22 in association with VP16 [0.03%]
病毒即时早期蛋白ICP22与VP16协同调节HSV-1增强子α基因的转录
Wei Cun,Lei Guo,Ying Zhang et al.
Wei Cun et al.
Herpes Simplex Virus 1 (HSV1) is capable of inducing two forms of infection in individuals, and the establishment of which type of infection occurs is linked to the transcriptional activation of viral alpha genes. One of the HSV1 alpha gene...
Viswanathan Chinnusamy,Jian-Kang Zhu
Viswanathan Chinnusamy
RNA-directed DNA methylation (RdDM) is a nuclear process in which small interfering RNAs (siRNAs) direct the cytosine methylation of DNA sequences that are complementary to the siRNAs. In plants, double stranded-RNAs (dsRNAs) generated by R...
Cong Ma,YuFei Liu,Lin He
Cong Ma
microRNAs (miRNAs) encode a novel class of small, non-coding RNAs that regulate gene expression post-trancriptionally. miRNAs comprise one of the major non-coding RNA families, whose diverse biological functions and unusual capacity for gen...
Fei Lan,Yang Shi
Fei Lan
Histone methylation is believed to play important roles in epigenetic memory in various biological processes. However, questions like whether the methylation marks themselves are faithfully transmitted into daughter cells and through what m...
Rui-Ming Xu
Rui-Ming Xu