XiaoLi Guo,YiSheng Zhu,YiXue Li et al.
XiaoLi Guo et al.
The cleavability of the hemagglutinin (HA) plays a major role in virulence of avian influenza viruses. Detailed analyses of the cleavage sequences and their evolution would give insights into the high pathogenicity of the H5N1 virus. HA seg...
Analysis of proteins that interact with nucleocapsid protein of SARS-CoV using 15-mer phage-displayed library [0.03%]
采用十五肽噬菌体展示文库分析SARS-CoV核衣壳蛋白相互作用的蛋白质
ZhengXue Liu,ZhanHui Wang,YingLe Liu et al.
ZhengXue Liu et al.
Analysis of proteins that interact with N protein of SARS-CoV using 15-mer phage-displayed library will help to explore the virus pathogenesis and to develop new drugs and vaccines against SARS. In this study, we cloned, expressed and purif...
Using maximum likelihood method to detect adaptive evolution of HCV envelope protein-coding genes [0.03%]
利用极大似然法检测HCV包膜蛋白编码基因的适应性进化
Wenjuan Zhang,Yuan Zhang,Yang Zhong
Wenjuan Zhang
Nonsynonymous-synonymous substitution rate ratio (d N /d S ) is an important measure for evaluating selective pressure based on the protein-coding sequences. Maximum likelihood (ML) method with codon-substitution models is a powerful statis...
Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain [0.03%]
由完整长度的互补DNA合成猪水疱病病毒原型毒株感染性病毒呼吸道合胞病毒感染小鼠模型的建立与应用研究
Haixue Zheng,Xiangtao Liu,Youjun Shang et al.
Haixue Zheng et al.
The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RN...
Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys [0.03%]
一种设计用于恒河猴的艾滋病DNA疫苗的免疫反应研究
Lishu Zhang,Jin Ningyi,Song Yingjin et al.
Lishu Zhang et al.
An effective HIV-1 vaccine will be the ultimate solution for the prevention of HIV/AIDS, though HAART plays important roles in treating the disease. In this study, a large-scale recombinant DNA plasmid containing a designed HIV-1 multi-epit...
Detection and analysis system for hybridization images of lab-in-a-tube microarray [0.03%]
管式实验室微阵列杂交图像的检测与分析系统
Quanjun Liu,Qin Zhou,Yunfei Bai et al.
Quanjun Liu et al.
A lab-in-a-tube microarray system is developed for sample inspection and signal detection by fabricating a flat transparent window cap of the Eppendorf tube. The oligonucleotide microarray is immobilized on the inner surface of the cap. A s...
Infection of SARS-CoV on juvenile and adult Brandt's vole Microtus brandtii [0.03%]
SARS冠状病毒对布兰特草原土拨鼠的感染试验研究
Hong Gao,Jingpian Peng,Wei Deng et al.
Hong Gao et al.
We studied the infectious effect of SARS-CoV virus on juvenile and adult Brandt's Vole (Microtus brandtii) by nasal cavity spraying method (CCID50 is 105.7). SARS virus caused serious deaths in adults. The death adults demonstrated hemorrha...
Yuhua Yuan,Changhao Bi,Ju Li et al.
Yuhua Yuan et al.
The failed attempt to express HIV-gp41 inE. coli led to the investigation of HIV-gp41 segments, which is responsible for the toxicity toE. coli cells. A series of deletion mutants containing different regions ofgp41 gene were constructed an...
The milestone of membrane protein research: Nobel Prize in Chemistry for 2003 [0.03%]
里程碑式的膜蛋白研究获奖：2003年诺贝尔化学奖揭晓
Wenlong Zhao,Senfang Sui
Wenlong Zhao
Genome sequencing and characterization analysis of a Beijing isolate of chicken coronavirus infectious bronchitis virus [0.03%]
北京鸡传染性支气管炎病毒株的基因测序及分析
Weiwu Jin,Chen Chen,Ying Zhang et al.
Weiwu Jin et al.
Avian infectious bronchitis virus (AIBV) is classified as a member of the genus coronavirus in the family coronaviridae. The enveloped virus has a positive-sense, single-stranded RNA genome of approximately 28 kilo-bases, which has a 5' cap...