Fabio Seiji Otsuka,Maria Concepción García Otaduy,Otaciro Rangel Nascimento et al.
Fabio Seiji Otsuka et al.
The study of continuous wave (cw) electron paramagnetic resonance (EPR) spectra still poses a challenge for very broad signals, especially when the spectrum extends over a large part of the accessible field range. The difficulties derive fr...
Site-directed spin label EPR studies of the structure and membrane interactions of the bacterial phospholipase ExoU [0.03%]
位点定向自旋标记EPR研究细菌磷脂酶ExoU的结构及其膜相互作用
Samantha L Gies,Maxx H Tessmer,Dara W Frank et al.
Samantha L Gies et al.
Site-directed spin labeling (SDSL) has been invaluable in the analysis of protein structure and dynamics, and has been particularly useful in the study of membrane proteins. ExoU, an important virulence factor in Pseudomonas aeruginosa infe...
EPR Viscometric Measurements Using a 13C-Labeled Triarylmethyl Radical in Protein-based Biotherapeutics and Human Synovial Fluids [0.03%]
基于蛋白质的生物疗法和人滑液中的13C标记的三芳基甲基自由基EPR流变测量
Murugesan Velayutham,Martin Poncelet,Jessica A Perini et al.
Murugesan Velayutham et al.
The viscosity measurements are of clinical significance for evaluation of the potential pathological conditions of biological lubricants such as synovial fluids of joints, and for formulation and characterization of peptide- and protein-bas...
Conformational investigation of the asymmetric periplasmic domains of E. coli LptB2FGC using SDSL CW EPR spectroscopy [0.03%]
利用SDSL连续波EPR光谱法研究大肠杆菌LptB2FGC不对称周质结构域的构象状态
Nicholas P Cina,Candice S Klug
Nicholas P Cina
The majority of pathogenic Gram-negative bacteria benefit from intrinsic antibiotic resistance, attributed primarily to the lipopolysaccharide (LPS) coating of the bacterial envelope. To effectively coat the bacterial cell envelope, LPS is ...
Spin-labeling Insights into How Chemical Fixation Impacts Glycan Organization on Cells [0.03%]
化学固定如何影响细胞上的糖基组织结构的自旋标记洞察力
Mohit Jaiswal,Trang T Tran,Jiatong Guo et al.
Mohit Jaiswal et al.
As new methods to interrogate glycan organization on cells develop, it is important to have a molecular level understanding of how chemical fixation can impact results and interpretations. Site-directed spin labeling technologies are well s...
Structural Dynamics of Protein Interactions Using Site-Directed Spin Labeling of Cysteines to Measure Distances and Rotational Dynamics with EPR Spectroscopy [0.03%]
利用定点自旋标记半胱氨酸通过EPR光谱测量距离和旋转动力学来研究蛋白质相互作用的结构动力学
Osha Roopnarine,David D Thomas
Osha Roopnarine
Here we review applications of site-directed spin labeling (SDSL) with engineered cysteines in proteins, to study the structural dynamics of muscle and non-muscle proteins, using and developing the electron paramagnetic resonance (EPR) spec...
Spectroscopically Orthogonal Labelling to Disentangle Site-Specific Nitroxide Label Distributions [0.03%]
光谱正交标记法解析定点硝基自由基标签的分布情况
Valentina Vitali,Katrin Ackermann,Gregor Hagelueken et al.
Valentina Vitali et al.
Biomolecular applications of pulse dipolar electron paramagnetic resonance spectroscopy (PDS) are becoming increasingly valuable in structural biology. Site-directed spin labelling of proteins is routinely performed using nitroxides, with p...
A Highly Ordered Nitroxide Side Chain for Distance Mapping and Monitoring Slow Structural Fluctuations in Proteins [0.03%]
用于蛋白质距离映射及监测慢结构波动的高度有序的硝基自由基侧链
Mengzhen Chen,Tamás Kálai,Duilio Cascio et al.
Mengzhen Chen et al.
Site-directed spin labeling electron paramagnetic resonance (SDSL-EPR) is an established tool for exploring protein structure and dynamics. Although nitroxide side chains attached to a single cysteine via a disulfide linkage are commonly em...
In Vivo Myelin Water Quantification Using Diffusion-Relaxation Correlation MRI: A Comparison of 1D and 2D Methods [0.03%]
基于扩散张量与横向弛豫时间关联的活体髓鞘含水量定量研究：一种一维方法和二维方法的比较
Sebastian Endt,Maria Engel,Emanuele Naldi et al.
Sebastian Endt et al.
Multidimensional Magnetic Resonance Imaging (MRI) is a versatile tool for microstructure mapping. We use a diffusion weighted inversion recovery spin echo (DW-IR-SE) sequence with spiral readouts at ultra-strong gradients to acquire a rich ...
Małgorzata Marjańska,Thomas Z Teisseyre,Nicholas W Halpern-Manners et al.
Małgorzata Marjańska et al.
Recently, hyperpolarized substrates generated through dynamic nuclear polarization have been introduced to study in vivo metabolism. Injection of hyperpolarized [1-13C]pyruvate, the most widely used substrate, allows detection of time cours...