Presynaptic calcium concentration microdomains and transmitter release [0.03%]
presynaptic钙浓度微域与递质释放
R Llinás,M Sugimori,R B Silver
R Llinás
n-Aequorin J, a luminescent protein which responds to calcium concentration changes in the order of several hundred micromoles, was injected into the preterminal fiber in the squid giant synapse. The activation of the presynaptic terminal l...
Presynaptic calcium signals during neurotransmitter release: detection with fluorescent indicators and other calcium chelators [0.03%]
荧光指示剂和其他钙螯合剂在神经递质释放过程中检测突触前钙信号中的应用
G J Augustine,E M Adler,M P Charlton et al.
G J Augustine et al.
Synthetic calcium buffers, including fluorescent calcium indicators, were microinjected into squid 'giant' presynaptic nerve terminals to investigate the calcium signal that triggers neurotransmitter secretion. Digital imaging methods, appl...
G Liverini,S Iossa,A Barletta
G Liverini
We measured mitochondrial protein mass as well as State 4 and 3 respiratory rates using different substrates in isolated liver mitochondria from 30-day cold-exposed rats. In addition, we measured the respiration under different conditions o...
Agonist-induced production of inositol phosphates in human airway smooth muscle cells in culture [0.03%]
人支气管平滑肌细胞中激动剂引起的肌醇磷酸盐的产生
N Marmy,W Durand-Arczynska,J Durand
N Marmy
Smooth muscle cells (SMC) from human bronchi were isolated by elastase treatment, subcultured, and characterized by their positive reaction with a monoclonal antibody against alpha-smooth muscle actin (alpha SMA). In each cell line tested, ...
P André,M Michel,C Schott et al.
P André et al.
We describe a new method to obtain rat aortic endothelial cells without contamination by vascular smooth muscle cells. The endothelial cells were characterized up to the 20th passage by low density lipoprotein incorporation, the absence of ...
Atrial natriuretic peptide during water deprivation or hemorrhage in rats. Relationship with arginine vasopressin and osmolarity [0.03%]
大鼠脱水或失血时的脑钠潮素的变化及其与血管加压素和渗透压的关系研究
M A Zongazo,A Carayon,F Masson et al.
M A Zongazo et al.
The concentrations of atrial natriuretic peptide (ANP) in atria, hypothalami and plasma were investigated in relation to the variations of the plasma endogenous immunoreactive arginine vasopressin (Ir-AVP) during water deprivation or hemorr...
Altered mechanical responses of malignant hyperthermic skeletal muscle during repetitive stimulation [0.03%]
反复电刺激恶性高热骨骼肌的力学反应改变
J H Williams,M Holland,C W Ward et al.
J H Williams et al.
This investigation examined the mechanical responses of malignant hyperthermic (MH) and normal porcine skeletal muscle to repetitive stimulation. Twitch and maximal tetanic tensions were not significantly different between muscle types. Ten...
M E Greenberg,M A Thompson,M Sheng
M E Greenberg
Cellular immediate early genes (IEGs) are a class of genes whose transcription is transiently activated within minutes of exposure of cells to a wide range of extracellular stimuli. In mature neurons IEG expression can be triggered by a var...
C D Rasmussen,K P Lu,R L Means et al.
C D Rasmussen et al.
Previous studies have indicated a role for the calcium receptor calmodulin in the control of eukaryotic cell proliferation. Using a molecular genetic approach in the filamentous fungus Aspergillus nidulans we have shown that CaM is required...
Carbachol-stimulated calcium entry in SH-SY5Y human neuroblastoma cells: which route? [0.03%]
卡巴胆碱诱发的人神经母细胞瘤细胞钙离子内流通道的研究
D G Lambert,S R Nahorski
D G Lambert
M3 muscarinic receptors expressed on SH-SY5Y human neuroblastoma cells are linked to phosphoinositide turnover and rises in [Ca2+]i. The rise in [Ca2+]i is biphasic with the peak phase being due to release from an intracellular Ins(1,4,5)P3...