Craig E Cameron,Jamie J Arnold,Laurie S Kaguni
Craig E Cameron
Understanding viral replication and transcription using single-molecule techniques [0.03%]
利用单分子技术了解病毒的复制与转录过程
Emmanuelle Pitre,Aartjan J W Te Velthuis
Emmanuelle Pitre
DNA and RNA viruses depend on one or more enzymes to copy and transcribe their genome, such as a polymerase, helicase, or exonuclease. Because of the important role of these enzymes in the virus replication cycle, they are key targets for a...
Joy Y Feng,Adrian S Ray
Joy Y Feng
The therapeutic targeting of the nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) of the Hepatitis C Virus (HCV) with nucleotide analogs led to a deep understanding of this enzymes structure, function and substrate specif...
Kenneth A Johnson,Tyler Dangerfield
Kenneth A Johnson
Nucleotide analogs are the cornerstone of direct acting antivirals used to control infection by RNA viruses. Here we review what is known about existing nucleotide/nucleoside analogs and the kinetics and mechanisms of RNA and DNA replicatio...
Inhibition of viral RNA-dependent RNA polymerases with clinically relevant nucleotide analogs [0.03%]
临床相关核苷类似物对病毒RNA依赖性RNA聚合酶的抑制作用
Kieran Maheden,Brendan Todd,Calvin J Gordon et al.
Kieran Maheden et al.
The treatment of viral infections remains challenging, in particular in the face of emerging pathogens. Broad-spectrum antiviral drugs could potentially be used as a first line of defense. The RNA-dependent RNA polymerase (RdRp) of RNA viru...
Watching the bacterial RNA polymerase transcription reaction by time-dependent soak-trigger-freeze X-ray crystallography [0.03%]
通过时间依赖的浸泡触发冷冻X射线晶体学观察细菌RNA聚合酶转录反应
Yeonoh Shin,Katsuhiko S Murakami
Yeonoh Shin
RNA polymerase (RNAP) is the central enzyme of gene expression, which transcribes DNA to RNA. All cellular organisms synthesize RNA with highly conserved multi-subunit DNA-dependent RNAPs, except mitochondrial RNA transcription, which is ca...
Ekaterina Knyazhanskaya,Marc C Morais,Kyung H Choi
Ekaterina Knyazhanskaya
Flaviviruses such as dengue, Japanese encephalitis, West Nile, Yellow Fever and Zika virus, cause viral hemorrhagic fever and encephalitis in humans. However, antiviral therapeutics to treat or prevent flavivirus infections are not yet avai...
Picornaviral 2C proteins: A unique ATPase family critical in virus replication [0.03%]
小核糖核酸病毒2C蛋白：对病毒感染起关键作用的独特的ATP酶家族
Pu Chen,Zhijian Li,Sheng Cui
Pu Chen
The 2C proteins of Picornaviridae are unique members of AAA+ protein family. Although picornavirus 2C shares many conserved motifs with Super Family 3 DNA helicases, duplex unwinding activity of many 2C proteins remains undetected, and high...
Structural basis of viral RNA-dependent RNA polymerase nucleotide addition cycle in picornaviruses [0.03%]
小核糖核酸病毒属病毒的RNA依赖的RNA聚合酶核苷酸添加循环的结构基础
Peng Gong
Peng Gong
RNA-dependent RNA polymerases (RdRPs) encoded by RNA viruses represent a unique class of processive nucleic acid polymerases, carrying out DNA-independent replication/transcription processes. Although viral RdRPs have versatile global struc...
Mohamad S Sotoudegan,Jamie J Arnold,Craig E Cameron
Mohamad S Sotoudegan
Stochastic outcomes of viral infections are attributed in large part to multiple layers of intrinsic and extrinsic heterogeneity that exist within a population of cells and viruses. Traditional methods in virology often lack the ability to ...