Quantitative structure-activity relationship study of iodinated analogues of trimetoquinol as highly potent beta 2-adrenoceptor ligands [0.03%]
三甲氧基喹oline碘化物作为高效β2-肾上腺素受体配体的定量构效关系研究
R Kumar,P Singh
R Kumar
Trimetoquinol and its derivatives, reported to be highly potent beta 2-adrenoceptor (beta 2-AR) and site-selective thromboxane A2/prostaglandin H2 (TP) receptor ligands are subjected to quantitative structure-activity relationship (QSAR) st...
Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gels [0.03%]
加热介导的SDS-PAGE胶中Coomassie Brilliant Blue蛋白染色及脱色的一种快速方法
B T Kurien,R H Scofield
B T Kurien
For the detection of proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoresis, Coomassie blue is used commonly on account of its simplicity and reliability. In this report we show that enhanced heat, in addition to dramaticall...
Erythrocyte sodium and Na+, K(+)-ATPase activity in untreated hypertensives and their first degree relatives [0.03%]
原发性高血压病人及其一级亲属红细胞钠及Na+,K+-ATP酶活性变化的研究
K Sudhakar,M Sujatha,C V Devi et al.
K Sudhakar et al.
In this paper we report the erythrocyte sodium concentration and Na+, K(+)-ATPase activity in 86 untreated hypertensives and their 77 first degree relatives and also in sex and age matched controls. There was significant increase in erythro...
R Das Gupta,P K Ghosh,B Bano
R Das Gupta
Earlier, we had reported purification of three thiol proteinase inhibitors (TPI-1 of 70 kDa, TPI-3 of 195 kDa and TPI-4 of 497 kDa) from human plasma. In the present study we report that TPI-1 binds to papain in the stoichiometry ratio (E/I...
Decreased phospholipase A2 activity in butyrate differentiated HT29 cells [0.03%]
丁酸盐分化HT29细胞中磷脂酶A2活性降低
M Madesh,O Benard,K A Balasubramanian
M Madesh
Our earlier work has shown that in butyrate differentiated colonic HT29 cells, there is an alteration in phospholipid composition as compared to control. To know more about these changes, butyrate treated and control cell homogenates were i...
Regional effects of ageing on Na+,K(+)-ATPase activity in rat brain and correlation with multiple unit action potentials and lipid peroxidation [0.03%]
老龄对大鼠脑Na+,K(+)-ATP酶活性的区域性影响及其与多单位动作电位和脂质过氧化的相关性研究
J Kaur,D Sharma,R Singh
J Kaur
Effects of ageing on Na+,K(+)-ATPase activity in crude synaptosomal fractions from the rat brain parietal cortex, hippocampus, striatum and thalamus has been studied. From 12 months to 24 months, a progressive decline in enzyme activity in ...
A Chitre,S Padmanabhan,N V Shastri
A Chitre
Plants of the genus Dieffenbachia, very popular as indoor ornamental plants, are known for their toxic as well as therapeutic properties. Their toxic manifestations have been partly attributed to their proteolytic activity. The work describ...
Oxidation of phenols by horseradish peroxidase and lactoperoxidase compound II--kinetic considerations [0.03%]
芥子粉过氧化物酶和乳过氧化物酶复合物II对酚的氧化作用——动力学考虑
M S Zahida,W Deva,G M Peerzada et al.
M S Zahida et al.
Oxidation of para substituted phenols by horseradish peroxidase compound II (HRP-II) and lactoperoxidase compound II (LPO-II) were studied using stopped flow technique. Apparent second order rate constants (kapp) of the reactions were deter...
Purification and characterizaton of plastidic pyruvate kinase from developing seeds of Brassica campestris L [0.03%]
芸苔种子发育过程中丙酮酸激酶的分离和纯化
D K Singh,S P Malhotra,R Singh
D K Singh
Plastidic pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) was purified to near homogeneity as judged by native PAGE with about 4% recovery from developing seeds of Brassica campestris using (NH4)2SO4 fractionation, DEAE-cell...
Chemical modification studies of Rhizomucor miehei protease: evidence for the role of basic amino acids in enzyme catalysis [0.03%]
Rhizomucor miehei 蛋白酶的化学修饰研究：碱性氨基酸在酶促反应中的作用证据
P Shridas,R Boopathy
P Shridas
The effect of chemical modification on milk clotting and proteolytic activities of aspartyl protease obtained from Rhizomucor miehei NRRL 3500 was examined in the absence and the presence of its specific inhibitor pepstatin A. The effect on...