The enzymatic reaction catalyzed by lactate dehydrogenase exhibits one dominant reaction path [0.03%]
乳酸脱氢酶催化的反应主要沿一条路径进行
Jean E Masterson,Steven D Schwartz
Jean E Masterson
Enzymes are the most efficient chemical catalysts known, but the exact nature of chemical barrier crossing in enzymes is not fully understood. Application of transition state theory to enzymatic reactions indicates that the rates of all pos...
A Molecular Dynamics Simulation Study of the Association of 1,1'-Binaphthyl-2,2'-diyl hydrogenphosphate Enantiomers with a Chiral Molecular Micelle [0.03%]
取向分子动力学模拟研究联萘酚光学异构体与手性胶束体系的相互作用
Kevin F Morris,Eugene J Billiot,Fereshteh H Billiot et al.
Kevin F Morris et al.
Molecular dynamics (MD) simulations were used to investigate the binding of 1,1'-binaphthyl-2,2'-diyl hydrogenphosphate (BNP) enantiomers to the molecular micelle poly-(sodium undecyl-(L,L)-leucine-valine) (poly(SULV)). Poly(SULV) is used a...
Real-time tracking of CO migration and binding in the α and β subunits of human hemoglobin via 150-ps time-resolved Laue crystallography [0.03%]
利用150皮秒时间分辨Laue晶体学实时跟踪人血红蛋白α和β亚基中CO的迁移和结合过程
Friedrich Schotte,Hyun Sun Cho,Jayashree Soman et al.
Friedrich Schotte et al.
We have developed the method of picosecond Laue crystallography and used this capability to probe ligand dynamics in tetrameric R-state hemoglobin (Hb). Time-resolved, 2 Å-resolution electron density maps of photolyzed HbCO reveal the time...
Simplified and economical 2D IR spectrometer design using a dual acousto-optic modulator [0.03%]
使用双声光调制器的简化经济型2D红外光谱仪设计
David R Skoff,Jennifer E Laaser,Sudipta S Mukherjee et al.
David R Skoff et al.
Over the last decade two-dimensional infrared (2D IR) spectroscopy has proven to be a very useful extension of infrared spectroscopy, yet the technique remains restricted to a small group of specialized researchers because of its experiment...
Structural Consequences of Chromophore Formation and Exploration of Conserved Lid Residues amongst Naturally Occurring Fluorescent Proteins [0.03%]
荧光蛋白构象变化及保守残基对其性质的影响研究
Matthew H Zimmer,Binsen Li,Ramza S Shahid et al.
Matthew H Zimmer et al.
Computational methods were used to generate the lowest energy conformations of the immature precyclized forms of the 28 naturally occurring GFP-like proteins deposited in the pdb. In all 28 GFP-like proteins, the beta-barrel contracts upon ...
EPFR Formation from Phenol adsorption on Al2O3 and TiO2: EPR and EELS studies [0.03%]
苯在Al2O3和TiO2表面吸附形成EPFR的研究：EPR与EELS方法
Matthew C Patterson,Nathan D Keilbart,Lucy W Kiruri et al.
Matthew C Patterson et al.
We have examined the formation of environmentally persistent free radicals (EPFRs) from phenol over alumina and titania using both powder and single-crystal samples. Electron paramagnetic resonance (EPR) studies of phenol adsorbed on metal ...
Measuring ultrafast protein folding rates from photon-by-photon analysis of single molecule fluorescence trajectories [0.03%]
基于单分子荧光轨迹的光子分析测量超快蛋白质折叠速率
Hoi Sung Chung,Troy Cellmer,John M Louis et al.
Hoi Sung Chung et al.
Folding and unfolding rates for the ultrafast folding villin subdomain were determined from a photon-by-photon analysis of fluorescence trajectories in single molecule FRET experiments. One of the obstacles to measuring fast kinetics in sin...
Using D-Amino Acids to Delineate the Mechanism of Protein Folding: Application to Trp-cage [0.03%]
以D型氨基酸揭示蛋白质折叠机理的研究——Trp-cage蛋白折叠研究为例
Robert M Culik,Srinivas Annavarapu,Vikas Nanda et al.
Robert M Culik et al.
Using the miniprotein Trp-cage as a model, we show that D-amino acids can be used to facilitate the delineation of protein folding mechanism. Specifically, we study the folding-unfolding kinetics of three Trp-cage mutants where the native g...
Nanosecond Dynamics of InfluenzaA/M2TM and an Amantadine Resistant Mutant Probed by Time-Dependent Red Shifts of a Native Tryptophan [0.03%]
纳伏时间范围内胺苔丁耐药性变异株和流感病毒A/M2TM的动态变化规律及.native色氨酸的时间依赖红移性质研究
Vikas Nanda,Lidia Cristian,Dmitri Toptygin et al.
Vikas Nanda et al.
Proteins involved in functions such as electron transfer or ion transport must be capable of stabilizing transient charged species on time scales ranging from picoseconds to microseconds. We study the influenza A M2 proton channel, containi...
Simulations of Two-dimensional Infrared and Stimulated Resonance Raman Spectra of Photoactive Yellow Protein [0.03%]
光活性黄色蛋白的二维红外及受激共振拉曼光谱模拟研究
Nicholas K Preketes,Jason D Biggs,Hao Ren et al.
Nicholas K Preketes et al.
We present simulations of one and two-dimensional infrared (2DIR) and stimulated resonance Raman (SRR) spectra of the dark state (pG) and early red-shifted intermediate (pR) of photoactive yellow protein (PYP). Shifts in the amide I and Glu...