Optimising cryopreservation protocols for haematopoietic progenitor cells: a methodological approach for umbilical cord blood [0.03%]
优化造血祖细胞冷冻保存方法的方案及应用于脐带血的方法学研究
Charles J Hunt,David E Pegg,Susan E Armitage
Charles J Hunt
Current cryopreservation protocols for haematopoietic cells have developed largely empirically and there is no consensus on an optimal method of preservation. These protocols, though providing sufficient cells to permit engraftment, can lea...
Characteristics of Atropa belladonna hairy roots cryopreserved by vitrification method [0.03%]
番红花根毛型培养物的玻璃化冷冻保存特性研究
Kaori Touno,Kayo Yoshimatsu,Koichiro Shimomura
Kaori Touno
Atropa belladonna hairy roots (clone M8) were successfully cryopreserved by using the vitrification method. A. belladonna hairy root tips were precultured on a half strength of Murashige and Skoog (MS) solid medium with 0.1 mg per L 2,4-D o...
Paul Watson
Paul Watson
Editorial
Cryo letters. 2006 Jan-Feb;27(1):1-2. DOI: 2006
Ultra rapid freezing and vitrification of human embryos derived from abnormally fertilised zygotes [0.03%]
来源于受精异常的卵裂期人类胚胎的超快速玻璃化冷冻保存技术研究进展
I Molina,C C Duque,J Alfonso et al.
I Molina et al.
The present study was undertaken to compare the developmental capacity of human embryos derived from abnormally fertilised zygotes (1 PN, > 3 PN; 16-18 hours after ICSI) cryopreserved using two techniques: ultra rapid freezing and vitrifica...
Comparative Study
Cryo letters. 2006 Jan-Feb;27(1):59-64. DOI: 2006
Characteristics of fresh gander semen and its susceptibility to cryopreservation in six generations derived from geese inseminated with frozen-thawed semen [0.03%]
冻精人工授精六代鹅精液特征及冷冻特性的研究
Ewa Łukaszewicz
Ewa Łukaszewicz
The paper summarises seven years experiments designed to determine the effect of continuous insemination with frozen-thawed semen on fresh semen quality and sperm susceptibility to freezing stress in succeeding generations. During course of...
Freeze-drying of human platelets: influence of intracellular trehalose and extracellular protectants [0.03%]
人血小板的冻干：细胞内海藻糖和细胞外保护剂的影响
Xin-Li Zhou,Hong Zhu,Shao-Zhi Zhang et al.
Xin-Li Zhou et al.
Freeze-drying is an ideal alternative for long-term preservation of platelets in blood banks. Intracellular trehalose is believed to be an effective lyoprotectant for preserving cells during freeze-drying. In this study, 13 mM intracellular...
Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification [0.03%]
包裹脱水和玻璃化法 cryo保存黑莓和树头菜的芽尖
Sandhya Gupta,Barbara M Reed
Sandhya Gupta
Encapsulation-dehydration and PVS2-vitrification cryopreservation protocols were evaluated for the long-term conservation of a diverse group of Rubus germplasm. Cold acclimation for a 4-week period prior to cryopreservation was necessary fo...
Comparative Study
Cryo letters. 2006 Jan-Feb;27(1):29-42. DOI: 2006
Characterisation of cryopreserved cells freshly isolated from human bone marrow [0.03%]
人骨髓中低温保存的细胞的表征属性特征描述属性标化属性刻画形象性格品格品行品德人格特色特点特性形象形象形象
Philippa A Rust,Costas Tingerides,Stephen R Cannon et al.
Philippa A Rust et al.
Osteoblast progenitor cells (OBPCs) isolated from bone marrow have the ability to differentiate into osteoblasts and thus potential therapeutic use to tissue-engineer bone. In order for OBPCs to be available for clinical use a means of stor...
Comparative Study
Cryo letters. 2006 Jan-Feb;27(1):17-28. DOI: 2006
Cryopreservation, encapsulation and promotion of shoot production of embryonic axes of a recalcitrant species Ekebergia capensis, Sparrm [0.03%]
胚轴休眠、包埋及难结种子植物Ekebergia capensis Sparrm幼苗产生的促进与超低温保存
Rosa Perán,Patricia Berjak,N W Pammenter et al.
Rosa Perán et al.
A study on zygotic axes of the recalcitrant seeds of Ekebergia capensis compared two cryopreservation methods, partial desiccation, and encapsulation-dehydration, and also investigated a method to promote shoot production. High (80 percent)...
Letter to the editor: analysis of thermo-mechanical stress in cryopreservation [0.03%]
读者来信：冷冻保存中的热机械应力分析
Yeod Rabin,Paul S Steif
Yeod Rabin