Mapping of excitatory and inhibitory postsynaptic potentials of neuronal populations in hippocampal slices using the GEVI, ArcLight [0.03%]
基于GEVI ArcLight在海马脑片上群体神经元的兴奋性和抑制性突触后电位映射研究
Ryuichi Nakajima,Bradley J Baker
Ryuichi Nakajima
To understand the circuitry of the brain, it is essential to clarify the functional connectivity among distinct neuronal populations. For this purpose, neuronal activity imaging using genetically-encoded calcium sensors such as GCaMP has be...
Patricia Bassereau,Rui Jin,Tobias Baumgart et al.
Patricia Bassereau et al.
The importance of curvature as a structural feature of biological membranes has been recognized for many years and has fascinated scientists from a wide range of different backgrounds. On the one hand, changes in membrane morphology are inv...
S Richter,R Gerum,A Winterl et al.
S Richter et al.
Emperor penguins (Aptenodytes forsteri) are highly adapted to the harsh conditions of the Antarctic winter: they are able to fast for up to 134 days during breeding. To conserve energy, emperor penguins form tight groups (huddles), which is...
Direct AFM Visualization of the Nanoscale Dynamics of Biomolecular Complexes [0.03%]
直接观察生物分子复合物的纳米尺度动态行为原子力显微镜可视化研究
Yuri L Lyubchenko
Yuri L Lyubchenko
High-speed AFM (HS-AFM) is an advanced technique with numerous applications in biology, particularly in molecular biophysics. Developed as a time-lapse AFM technique for direct imaging fully hydrated biological molecules, HS-AFM is currentl...
Richard Gerum,Sebastian Richter,Ben Fabry et al.
Richard Gerum et al.
During breeding, king penguins do not build nests, however they show strong territorial behaviour and keep a pecking distance to neighbouring penguins. Penguin positions in breeding colonies are highly stable over weeks and appear regularly...
Complementary studies of lipid membrane dynamics using iSCAT and super-resolved fluorescence correlation spectroscopy [0.03%]
基于iSCAT和超分辨率荧光相关光谱技术的脂膜动态学互补研究
Francesco Reina,Silvia Galiani,Dilip Shrestha et al.
Francesco Reina et al.
Observation techniques with high spatial and temporal resolution, such as single-particle tracking based on interferometric scattering (iSCAT) microscopy, and fluorescence correlation spectroscopy applied on a super-resolution STED microsco...
Linda Oswald,Steffen Grosser,David M Smith et al.
Linda Oswald et al.
The traditional picture of tissues, where they are treated as liquids defined by properties such as surface tension or viscosity has been redefined during the last few decades by the more fundamental question: under which conditions do tiss...
Biological sensing and control of emission dynamics of quantum dot bioconjugates using arrays of long metallic nanorods [0.03%]
利用金属纳米线阵列生物传感和控制量子点复合物发射动力学
Seyed M Sadeghi,Rithvik R Gutha,Waylin J Wing et al.
Seyed M Sadeghi et al.
We study biological sensing using plasmonic and photonic-plasmonic resonances of arrays of ultralong metallic nanorods and analyze the impact of these resonances on emission dynamics of quantum dot bioconjugates. We demonstrate that the LSP...
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane [0.03%]
Laurdan和Di-4-ANEPPDHQ膜探针检测膜的不同性质
Mariana Amaro,Francesco Reina,Martin Hof et al.
Mariana Amaro et al.
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing. However, detailed insights into ...
Vukica Šrajer,Marius Schmidt
Vukica Šrajer
Macromolecular crystallography was immensely successful in the last two decades. To a large degree this success resulted from use of powerful third generation synchrotron X-ray sources. An expansive database of more than 100,000 protein str...