A HaloTag-Based Reporter System Enabling Two-Color Timestamped Gene Expression Monitoring [0.03%]
基于HaloTag的双色报告系统实现基因表达的时间记录监测
Henriette Lämmermann
Henriette Lämmermann
Precise gene expression regulation is essential for cellular homeostasis, particularly in stress response pathways. In the endoplasmic reticulum, the unfolded protein response (UPRER) plays an important role in maintaining the protein foldi...
Eva Freisinger,Michal Juríček,Hans Peter Lüthi
Eva Freisinger
Editorial
Chimia. 2026 Apr 29;80(4):201. DOI:10.2533/chimia.2026.201 2026
Unlocking the Multifaceted Role of CB1R in the Endocannabinoid System with Labeled Chemical Probes [0.03%]
利用放射性标记化学探针揭示内源大麻素系统中CB1R的多种功能作用
Leonard Mach,Marc Nazare
Leonard Mach
The endocannabinoid system is a key homeostatic regulator that influences multiple physiological processes across nervous, immune, and metabolic systems in humans. Despite decades of pharmaceutical research, only a few clinically relevant o...
Expanding The Scope of AKT Modulation Through Targeted Protein Degradation [0.03%]
靶向蛋白降解扩大AKT通路调节范围
Marco Serafini,Rui Moreira
Marco Serafini
AKT is a critical mediator of the phosphoinositide 3-kinase (PI3K) signalling cascade, playing a key role in regulating essential cellular processes. The identification of AKT as one of the most dysregulated pathways in cancer led to the de...
Yang Liu,Peng Wu
Yang Liu
IRE1α is an important ER stress sensor located on the ER membrane with dual kinase and ribonuclease activity. It plays a crucial role in restoring ER proteostasis and is associated with various human diseases. Targeting IRE1α has become a...
Multiplexed Gel-based ABPP Strategy for Target-agnostic Serine Hydrolase Inhibitor Screening [0.03%]
一种用于筛选靶点不可知丝氨酸水解酶抑制剂的凝胶基ABPP多重检测策略
Franciscus H G Ter Brake,Patrick Rehorst,Machiel W Henst et al.
Franciscus H G Ter Brake et al.
Serine hydrolases (SHs) represent a functionally defined enzyme class with significant roles in human physiology, yet many of the approximately 240 human SH's lack selective chemical inhibitors required for functional validation. To address...
Joanna Bonnici,Christopher J Schofield,Akane Kawamura
Joanna Bonnici
Jumonji C histone lysine demethylases (JmjC-KDMs) are key chromatin regulators best known for catalysing histone lysine demethylation. There is growing evidence that JmjC-KDMs have a broader catalytic scope. This review summarises recent ad...
Dominic Scopelliti,Changfeng Deng,Benjamin A Garcia et al.
Dominic Scopelliti et al.
Protein arginylation is a conserved post-translational modification in eukaryotes, involving the conjugation of arginine residues to proteins by the enzyme arginyl-tRNA transferase. Historically associated with targeted degradation, recent ...
Martin Bitsche,Malou Hanisch,Alexandra Lusser et al.
Martin Bitsche et al.
The fluorogen-activating aptamer, Spinach, was a milestone in cellular RNA imaging and conceptually similar to the green fluorescent protein (GFP). Since then, more than ten FLAP systems have been developed across a broad spectral range. Al...
Nanopore Studies of DNA Damage and G-quadruplex Folding in the Human Telomere Sequence [0.03%]
人类端粒序列中DNA损伤和G-四链体折叠的纳米孔研究
Aaron M Fleming,Cynthia J Burrows
Aaron M Fleming
The bacterial pore-forming toxin α-hemolysin has dimensions appropriate for capture and translocation of DNA strands in a single-molecule electrophoresis experiment. We used the nanopore's properties to study G-quadruplex unfolding in the ...