Juncheng Liu,Bing Xu,Mark A R de Geus et al.
Juncheng Liu et al.
Although it is well-known that ionizing radiation generates hydrogen peroxide in aqueous solution, using this approach to activate boronic acid or ester-based prodrugs suffers from low H2O2 yields and thus low uncaging efficiency....However, the organochloride peroxyl radical formed from irradiating an aqueous solution of an organochloride may increase the uncaging efficiency.
Guoming Tong,Changfeng Li,Qing Zhou et al.
Guoming Tong et al.
Spatiotemporal precision in photocontrolled biochemical reactions remains a central challenge. We report a sequential activation platform that integrates biomarker-triggered unlocking of photocatalyst activity with photocatalytic bioorthogo...
Ionotropic glutamate receptor function in interpeduncular nucleus is modulated by nicotine exposure [0.03%]
尼古丁暴露通过调控中间 pedunculocommissural 核区离子型谷氨酸受体功能诱导小鼠焦虑行为
Yijin Yan,Brenton R Tucker,Andrew E Tapp et al.
Yijin Yan et al.
After confirming mRNA expression of Gria1 and Grin1 iGluR subunits in IPR, we employed glutamate uncaging coupled with 2-photon imaging and patch clamp electrophysiology.
Optical control of PI(4,5)P2 sensitivity of ion channels by manipulation of single lysine residue [0.03%]
通过操纵单赖氨酸残基对离子通道进行光学控制以调控PI（4,5）P2敏感性
Junxian Zhou,Rizki Tsari Andriani,Natsuki Mizutani et al.
Junxian Zhou et al.
Caging of lysine by introducing HCK at K182 or K187 completely silenced Kir2.1 currents, but light-induced uncaging restored current activity. Voltage-sensing phosphatase assays revealed that this current increase was accompanied by enhanced PI(4,5)P2 sensitivity....On the other hand, introducing HCK at K219, which forms a secondary PI(4,5)P2-binding region, did not fully eliminate Kir2.1 currents, and uncaging resulted in an approximately twofold increase in current....Analysis of uncaging and PI(4,5)P2 sensitivity in Kir2.1-K219HCK revealed that the region C-terminal to residue K219 is dispensable when assembled with the full-length protein.
A Picolyl-Based Cys Caging/Uncaging Strategy Facilitates Protein Synthesis [0.03%]
基于Picolyl的半胱氨酸修饰方法促进蛋白质合成
Farong Ye,Hanxi Bai,Xinliang Liu et al.
Farong Ye et al.
To address this challenge, we developed a novel and simple picolyl-based PPG for Cys caging/uncaging, which enables rapid orthogonal caging of thiols and their subsequent uncaging via pH and wavelength control.
Decoding ultrasensitive self-assembly of the calcium-regulated Tetrahymena cytoskeletal protein Tcb2 using optical actuation [0.03%]
基于光作用的调控使钙离子激活的变形虫细胞骨架蛋白Tcb2自组装过程变得清晰
Nithesh P Chandrasekharan,Xiangting Lei,Jerry Honts et al.
Nithesh P Chandrasekharan et al.
Light-driven uncaging of the photolabile calcium chelator DMNP-EDTA stimulates rapid localized self-assembly of Tcb2 into micron-scale gel-like protein networks.
Sparse innervation and local heterogeneity in the vibrissal corticostriatal projection [0.03%]
稀疏传入和局部异质性在触须皮层纹状体投射中的作用
Kenza Amroune,Lorenzo Fontolan,Agnès Baude et al.
Kenza Amroune et al.
Using ex vivo patch-clamp recordings in the DLS and glutamate uncaging for focal stimulations in the barrel cortex, we were able to map the location of presynaptic neurons to individual striatal projection neurons (SPNs).
NIR-Activatable, Sequence-Specific Metal-Nucleic Acid Scaffolds for Responsive Uncaging [0.03%]
用于响应性脱保护的近红外激活、序列特异性金属核酸支架
Arpit Sharma,Man Kshetri,Deepak Karna et al.
Arpit Sharma et al.
Using two Pt(IV)-caged fluorophores (MCA and BDP), we demonstrate efficient uncaging and high sequence specificity in both solution and live cells.
Erik Schrunk,Sunho Lee,Przemysław Dutka et al.
Erik Schrunk et al.
Photo-uncaging─the use of light to reveal the active part of a chemical compound by photolysis of a protecting group─has long been used to study and actuate biochemical processes. However, light scattering limits the applications of photo...
Submembrane liprin-α1 clusters spatially localize insulin granule fusion [0.03%]
底膜脂蛋白通过空间定位调控胰岛素囊泡融合释放反应
Kylie Deng,Kitty Sun,Nicole Hallahan et al.
Kylie Deng et al.
Using glucose or high K+ stimulation or the global uncaging of Ca2+, we show granule fusion consistently focused to the β cell-ECM interface, suggesting a specific localization mechanism. We tested for the involvement of liprin-α1, a scaffold protein enriched at the β cell-ECM interface.
耗时 0.08636 秒，为您在
49055432
条记录里面共找到 960 篇文章 [XML]