The American Society for Microbiology's evidence-based laboratory medicine practice guidelines for the diagnosis of bloodstream infections using rapid tests: a systematic review and meta-analysis [0.03%]
美国微生物学会关于使用快速试验诊断血流感染的基于证据的实验室实践指南：系统评价和meta分析
Donna M Wolk,J Scott Parrott,N Esther Babady et al.
Donna M Wolk et al.
Rapid identification of pathogens and detection of a few resistance markers from positive blood cultures are now possible through the increased availability of commercial rapid diagnostic tests, including nucleic acid amplification tests and matrix-assisted laser desorption ionization time-of-flight...Keywords: blood culture; bloodstream infection; diagnostics; guidelines; mass spectrometry; medical outcomes; nucleic acid amplification test; rapid tests; stewardship.
Flexible Delivering Potential of Oligonucleotides Reconstructed DNA Tetrahedron [0.03%]
基于DNA四面体的寡核苷酸传输载体的研究进展与挑战
Bicai Tang,Yong Li,Qiumei Li et al.
Bicai Tang et al.
Tetrahedral framework nucleic acid (tFNA), a 3D nanostructure formed by four single-stranded DNAs (ssDNAs), has shown great application potential for excellent editability, good biocompatibility, abundant bio-functions, and superior drug loading capacity.
Optimizing mouse metatranscriptome profiling by selective removal of redundant nucleic acid sequences [0.03%]
通过选择性去除冗余核酸序列优化小鼠元转录组分析
Morgan Roos,Samuel Bunga,Asako Tan et al.
Morgan Roos et al.
Metatranscriptome (MetaT) sequencing is a critical tool for profiling the dynamic metabolic functions of microbiomes. In addition to taxonomic information, MetaT also provides real-time gene expression data of both host and microbial popula...
Modified Bis-pyrimidine Clamps for Triplex Formation and Their Use in SARS-CoV‑2 Detection [0.03%]
用于三链形成和SARS-CoV-2检测的改良型双嘧啶钳锁核酸
Arnau Domínguez,Raimundo Gargallo,Carlos Cuestas-Ayllón et al.
Arnau Domínguez et al.
The formation of nucleic acid triple helices ("triplexes") is an area of great interest due to their potential role in the natural and artificial regulation of gene expression or for use in analytical, diagnostic, or synthetic methods.
Case Report: Positive Mycoplasma pneumoniae IgM does not necessarily indicate acute infection: two case studies [0.03%]
病例报告：肺炎支原体IgM阳性不一定代表急性感染：两个病例研究
Hao Wang,Xiaoying Liu,Yabin Wu et al.
Hao Wang et al.
Quantitative PCR was used to detect M. pneumoniae in bronchoalveolar lavage fluid and antigen tests and nucleic acid detection were conducted for other respiratory pathogens.
[Changes of hemagglutinin gene characteristics of influenza virus A(H3N2) during the 2022-2024 influenza season in Beijing] [0.03%]
2022—2024年北京市流感病毒A（H3N2）亚型血凝素基因特征变化分析
D T Zhang,X M Peng,L Zhang et al.
D T Zhang et al.
During the influenza seasons of 2022-2024, the positive rates of A(H3N2) nucleic acid were 10.35% (2 127/20 543) and 10.47% (4 386/41 876), respectively. In the 2022-2023 influenza season, there were two peaks in the A(H3N2).
Morphological Bone Score as a Predictive Tool for Molecular Profiling Success [0.03%]
形态骨评分作为分子谱分析成功的预测工具
Kirill Kriukov,Dmitry Ivchenkov,Anna Bejanyan et al.
Kirill Kriukov et al.
However, it can lead to nucleic acid degradation, resulting in next-generation sequencing (NGS) failures that impede diagnostic solutions for patients....The MBS, constructed using five key morphological features, assigns scores from 0 to 11, reflecting low to high tissue damage and direct proportionality with nucleic acid yields per cell.
Veerta Sharma,Reet Verma,Prateek Sharma et al.
Veerta Sharma et al.
In NDDs, the accumulation of misfolded or mutant proteins compromises mitochondrial function, leading to the release of mitochondrial or nuclear deoxyribose nucleic acid (DNA) into the cytosol.
DNA triangular prism nanostructure and CRISPR/Cas12a empowered electrochemical biosensor for dual detection of alpha-fetoprotein and microRNA 122 [0.03%]
用于同时检测甲胎蛋白和微小核糖核酸122的基于DNA三角棱柱纳米结构及CRISPR/Cas12a的电化学生物传感器
Xuemei Zhang,Li Zhu,Li Yang et al.
Xuemei Zhang et al.
The rigid, stable, and size-controllable DTP nano-framework enhanced nucleic acid hybridization and signal molecule binding while minimizing nonspecific adsorption, eliminating masking agent requirements, with its ordered assembly facilitating CRISPR/Cas12a access to ssDNA for improved cleavage efficiency
Supercharged DNA origami enhanced signal amplification for ultrasensitive detection of nucleic acid [0.03%]
超充电DNA折纸增强信号放大用于核酸 ultra灵敏检测
Weiwei Qin,Xueyan Gong,Xueyuan Duan et al.
Weiwei Qin et al.
In this paper, we proposed a portable electrochemical sensor for the detection of nucleic acid targets, using circulating tumor DNA (ctDNA) as an example. This biosensor combined DNA origami with high-curvature gold nanostructures.
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