Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus [0.03%]
利用农杆菌菌株82.139的T-DNA基因共转化增强转基因杨树植株的再生能力
Greg S Goralogia,Cathleen Ma,David S Taylor et al.
Greg S Goralogia et al.
Further mutational analysis of each gene identified 6b, in combination with iaaH, iaaM and ipt, as the major factor required for non-cell autonomous shoot proliferation.
Structural basis of the hepatitis B virus X protein in complex with DDB1 [0.03%]
与DDB1结合的乙肝病毒X蛋白复合物的结构基础
Hiroki Tanaka,Joao Diogo Dias,Basile Jay et al.
Hiroki Tanaka et al.
In this structure, hydrophobic interactions within HBx were identified, and mutational analysis highlighted their importance in the HBV life cycle.
Phenethyl Acetate as an Agonist of Insect Odorant Receptor Co-Receptor (Orco): Molecular Mechanisms and Functional Insights [0.03%]
苯乙酸作为昆虫气味受体共受体（Orco）的激动剂：分子机制及功能见解
Myungmi Moon,Jihwon Yun,Minsu Pyeon et al.
Myungmi Moon et al.
A mutational analysis identified W146 and E153 as critical residues for PA binding and activation. A double-mutant Orco receptor (W146A + E153A) exhibited a significant reduction in PA-induced activation compared to the wild-type receptor.
Natália G Quel,Leonardo T Rosa,Larissa M Antonio et al.
Natália G Quel et al.
However, mutational analysis revealed that ATPase activity requires both AaRUVBL1 and AaRUVBL2, in contrast to the human ortholog, where RUVBL1 and RUVBL2 alone are active ATPases. To characterize the structure of the AaRUVBL1/2 complex, we combined SAXS and Cryo-EM techniques.
Biochemical and structural characterization of a GNAT superfamily protein acetyltransferase from Helicobacter pylori [0.03%]
来源于幽门螺旋杆菌的GNAT超家族蛋白乙酰转移酶的生化及结构研究
Venkatareddy Dadireddy,Amrendra Kumar,Sumith Kumar et al.
Venkatareddy Dadireddy et al.
Structure-based mutational analysis showed that no general base is required for the enzymatic activity. However, a conserved catalytic water molecule at the active site is likely to serve the purpose.
Exploring the coupling of dynamic residues in TEM-1 β-lactamase and their role in activity [0.03%]
探究TEM-1β-内酰胺酶中动态残基的耦合及其在活性中的作用
Fatma Gizem Avci,Didem Vardar Ulu,Basak Atas Erden et al.
Fatma Gizem Avci et al.
Representative residues, K215, E104, E110, T114, and R43, were selected for mutational analysis to assess their roles in enzyme activity. Notably, the mutation of R43 to alanine led to a significant reduction in activity, with up to 70%.
Clinicopathologic and molecular mutational analysis of inflammatory fibroid polyps [0.03%]
炎症性纤维瘤息肉的临床病理和分子突变分析
Qianyun Shi,Biao Zhang,Jun Yang et al.
Qianyun Shi et al.
Immunohistochemistry of mesenchymal markers (including PDGFRα, DOG1, CD34, SMA, and et al.), and molecular mutational analysis of PDGFRA were carried out.
Wisteria floribunda agglutinin enhances Zaire ebolavirus entry through interactions at specific N-linked glycosylation sites on the virus glycoprotein complex [0.03%]
葛根素通过病毒糖蛋白复合体上特定的N-连接聚糖位点相互作用增强埃博拉病毒感染力
Joshua D Duncan,Monika Pathak,Barnabas J King et al.
Joshua D Duncan et al.
WFA was demonstrated to bind directly with the EBOV-GP via the glycans, and mutational analysis implicated N238 as contributing to the interaction. Furthermore, enhancement was observed in both human and bat cell lines, indicating a highly conserved mechanism of action.
Genetic Profiling Reveals the Distinctions Among MTX-Associated DLBCL, EBV-Positive Mucocutaneous Ulcer, and EBV + DLBCL [0.03%]
基因分析揭示了MTX相关DLBCL、EB病毒阳性黏膜皮肤溃疡与EB病毒阳性DLBCL之间的区别
Takumi Takahashi,Keisuke Sawada,Takahisa Yamashita et al.
Takumi Takahashi et al.
A mutational analysis has been performed on post-transplantation and HIV-positive lymphomas, but not on other iatrogenic immunodeficiency (OII)-associated LPDs mainly caused by methotrexate (MTX) to treat rheumatoid arthritis.
Dual synthesis pathways of scaRNA28 via intronic processing of transformation/transcription domain-associated protein transcripts and a novel independent transcription unit [0.03%]
转化/转录域相关蛋白转录本和新型独立转录单位的内含子加工生成scaRNA28的双重合成途径
Keiichi Izumikawa,Tatsuya Shida,Hideaki Ishikawa et al.
Keiichi Izumikawa et al.
Linker-scanning mutational analysis revealed that the promoter region required for scaRNA28 expression in the ITU is located within 60 bases including exon 2/intron 2 junction of TRRAP, and especially the first two bases of intron 2 region, a putative part of the MYC-binding (E-box) motif, are essential
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