Graphene Nanodots as Substrates for SEIRAS and SERS Studies on Membrane Proteins [0.03%]
石墨烯纳米点作为膜蛋白SEIRAS和SERS研究的衬底
Ariadna Murillo-Bello,Antonio Gaetano Ricciardulli,Abdul Rehman Umar et al.
Ariadna Murillo-Bello et al.
Graphene nanostructures are capable of supporting plasmonic resonances in the visible and infrared parts of the spectrum. Thus, they can be exploited as platforms for Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) and Surface-En...
Practical Tips for the Application of HDX-MS to Membrane Proteins, Biomolecular Condensates, and Weak Protein Binders [0.03%]
HDX-MS在膜蛋白、生物分子凝聚物和弱结合蛋白配体应用中的实用技巧
Xiaoxuan Lin,Andrew V Molina,Julia Shangguan et al.
Xiaoxuan Lin et al.
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful biophysical tool to study protein thermodynamics, dynamics, and function. Despite its increasing use, effective implementation of HDX-MS requires knowledge of ...
CRISPRi screening reveals E. coli's anaerobic-like respiratory adaptations to gentamicin: membrane depolarization by CpxR [0.03%]
利用CRISPR干扰筛选揭示的大肠杆菌对庆大霉素的无氧呼吸型适应：CpxR导致膜去极化
Donghui Choe,Eunju Lee,Yoseb Song et al.
Donghui Choe et al.
Our findings revealed that ribosomal proteins, ribosome-associated proteins, toxin-antitoxin systems, and outer membrane proteins strongly influence the fitness of E. coli in gentamicin.
A pH-responsive PEG coating strategy for enhancing the enrichment of small extracellular vesicles towards disease regions with acidic microenvironment [0.03%]
一种pH响应性PEG包覆策略：通过增强小型细胞外囊泡在具有酸性微环境的疾病区域的富集来改善药物递送
Jianwei Zhao,Xinyu Niu,Lei Luo et al.
Jianwei Zhao et al.
Western blot analysis and cellular uptake studies demonstrated that mPEG-CDM anchors to sEV membrane proteins through acid-labile cis-aconityl bonds, significantly reducing macrophage-mediated phagocytosis under physiological conditions, while restoring cellular internalization in endothelial cells (
Global and local effects in lipid-mediated interactions between peripheral and integral membrane proteins [0.03%]
与膜周边蛋白和内在蛋白的脂质介导相互作用中的全局效应及局部效应
Tatiana K Rostovtseva,David P Hoogerheide,William A Milhizer et al.
Tatiana K Rostovtseva et al.
Amphitropic proteins (APs) are a subfamily of water-soluble peripherally membrane-bound proteins that interact directly with the lipid membrane rather than with intrinsic membrane proteins and are therefore strongly influenced by membrane properties.
Protocol for the visualization and identification of S-palmitoylated proteins in HCT-116 cells using metabolic labeling via click chemistry [0.03%]
基于点击化学的代谢标记技术检测HT-116细胞内S-棕榈酰化蛋白质的实验方法学探索研究
Nadine Merz,Sabine Grösch
Nadine Merz
Further, we detail procedures for the quantification of S-palmitoylation of isolated membrane proteins via reporter fluorophore on the western-blot level without lipid background.
Polyvalent folate receptor-targeting chimeras for degradation of membrane proteins [0.03%]
一种用于降解膜蛋白的多价叶酸受体靶向嵌合体
Dian Xiao,Jingwen Dong,Fei Xie et al.
Dian Xiao et al.
Lysosome-targeting chimeras (LYTACs) represent a revolutionary targeted protein degradation technology. However, the advancement of LYTACs faces substantial challenges due to the limited diversity of lysosome-trafficking receptors. In this ...
Proton gradient controls the lateral rearrangement of inner membrane domains in response to membrane fluidizer stress in Mycobacterium smegmatis [0.03%]
在分枝杆菌中质子梯度控制着内膜结构域的侧向重组以响应膜流动胁迫
Malavika Prithviraj,F N U Shivangi,Joel S Freundlich et al.
Malavika Prithviraj et al.
IMD, enriched in the sub-polar regions of actively growing rod-shaped cell, contains many membrane proteins involved in cell envelope biosynthesis. Dibucaine, a membrane fluidizer, disrupts plasma membrane integrity and de-partitions the IMD from the sub-polar regions.
Astral-data-independent acquisition depicts the dynamic landscape of milk fat globule membrane proteins in yak colostrum, transitional milk, and mature milk [0.03%]
基于天体的数据独立获取描述了牦牛初乳、过渡奶和成熟奶中乳脂肪球膜蛋白的动态景观
Yunduan Wang,Daoliang Lan,Shunyang Liu et al.
Yunduan Wang et al.
The MFGM proteins are crucial for neonatal growth, immune regulation, and metabolic balance throughout mammalian life cycle. However, the composition and dynamic changes of MFGM proteins in yak colostrum (MC), transitional milk (MT), and ma...
Engineering Liposomes with Cell Membrane Proteins to Disrupt Melanosome Transfer between Cells [0.03%]
利用细胞膜蛋白工程脂质体干扰黑素小体胞间转移
Chunhuan Liu,Yuchun Liu,Changhu Xue et al.
Chunhuan Liu et al.
To achieve this, we incorporate keratinocyte cell membrane proteins into liposomes formed with microfluidics. Such functionalization enables the liposomes to selectively anchor to the surface of pigment globules, which transport melanosomes between cells.
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