"On-Off" electrochemical sensing toward mercury pollution in tea via exonuclease III triggered target recycling amplification [0.03%]
基于切端酶引发目标递归放大作用的“开-关”电化学检测茶叶中的汞污染方法
Lili Zhou,Guangyue Hou,Dan Xi et al.
Lili Zhou et al.
Mercury ion (Hg2+) pollution in tea poses significant risks to human health due to its high toxicity, bioaccumulation, and potential to cause neurological and kidney damage. Sensitive monitoring of Hg2+ in tea is therefore critical for food...
Machine learning-assisted washing-free detection of extracellular vesicles by target recycling amplification based fluorescent aptasensor for accurate diagnosis of gastric cancer [0.03%]
机器学习辅助的无洗涤外泌体检测技术在胃癌精确诊断中的应用研究——基于目标循环放大荧光适配子传感器的方法
Xinrui Gu,Zeyu Fan,Luying Lu et al.
Xinrui Gu et al.
Extracellular vesicles (EVs) are promising non-invasive biomarkers for cancer diagnosis. EVs proteins play a critical role in tumor progress and metastasis. However, accurately and reliably diagnosing cancers is greatly limited by single pr...
Affordable infectious pathogen detection using a dual-mode biosensor integrating exonuclease III-assisted target recycling amplification with high-throughput 96-well microplate format [0.03%]
一种基于外切酶III辅助目标重复放大与高通量96孔微板的低成本感染病原体检测生物传感器
Hamza Moustakim,Aziz Amine,Hasna Mohammadi
Hamza Moustakim
The ongoing challenge of infectious pathogens highlights the need for accurate and accessible methods to discern their genetic signatures, especially in resource-limited settings. In response to this crucial requirement, we introduce an aff...
Dual-mode aptasensor based on P-CeO2NR@Mxene and exonuclease I-assisted target recycling for malachite green detection [0.03%]
基于P-CeO2NR@Mxene和 exonuclease I 的目标循环放大适配子传感器的双模式检测孔雀石绿方法
Mingyue Ye,Xianfeng Lin,Jin Li et al.
Mingyue Ye et al.
Malachite green (MG) has been illicitly employed in aquaculture as a parasiticide, however, its teratogenic and carcinogenic effects pose a significant human health threat. Herein, a dual-mode colorimetric and electrochemical aptasensor was...
Silver/copper bimetallic nanoclusters integrating with cryonase-assisted target recycling amplification detection of Salmonella typhimurium [0.03%]
银/铜双金属纳米簇结合cryonase辅助目标循环放大检测伤寒沙门氏菌
Shouyi Dou,Mengyue Liu,Fengjuan Zhang et al.
Shouyi Dou et al.
An unsophisticated fluorescence-enabled strategy is brought forward to process the highly sensitive fluorescence detection of Salmonella typhimurium (S. typhimurium) which based on polyethyleneimine (PEI)-templated silver/copper nanocluster...
Construction of a Label-Free Ratiometric Biosensor Based on Target Recycling Amplification and Hg-ZnSe QDs for Assay of BChE and OPs [0.03%]
基于目标循环放大和Hg-ZnSe量子点的无标记比色生物传感器的构建及乙酰胆碱酯酶和有机磷水解检测
Jiabao Zhang,Mengke Wang,Jinying Liu et al.
Jiabao Zhang et al.
Herein, we constructed a label-free ratiometric fluorescence biosensing strategy for the determination of butyrylcholinesterase (BChE) activity and organophosphorus (OPs) concentration. BChE promoted the hydrolysis of iodized s-butyrylthioc...
Heating promoted super sensitive electrochemical detection of p53 gene based on alkaline phosphatase and nicking endonuclease Nt.BstNBI-assisted target recycling amplification strategy at heated gold disk electrode [0.03%]
基于加热金盘电极上碱性磷酸酶和切割核酸内切酶Nt.BstNBI靶标循环扩增策略促进p53基因的超灵敏电化学检测
Zhen-Zhen Mi,Hao-Cheng Hu,Jian-Jun Sun et al.
Zhen-Zhen Mi et al.
An ultrasensitive electrochemical biosensor for detecting p53 gene was fabricated based on heated gold disk electrode coupling with endonuclease Nt.BstNBI-assisted target recycle amplification and alkaline phosphatase (ALP)-based electrocat...
A highly sensitive competitive aptasensor for AFB1 detection based on an exonuclease-assisted target recycling amplification strategy [0.03%]
基于外切酶辅助目标循环放大策略的高灵敏度AFB1竞争型适体传感器
Hongyan Zhan,Si Yang,Chenxi Li et al.
Hongyan Zhan et al.
Aflatoxin B1 (AFB1) is a typical mycotoxin found in agricultural products, and poses a huge threat to both humans and animals. Accurate and rapid measurement of AFB1 is essential for environmental analysis and food safety. Based on molecula...
Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification [0.03%]
基于 exonuclease I 触发目标循环放大和适配体交联水凝胶的黄曲霉毒素B1可视化定量检测传感器
Mengyao Zheng,Hongmei Liu,Jin Ye et al.
Mengyao Zheng et al.
For the on-site detection of aflatoxin B1 (AFB1), a DNA hydrogel was prepared as a biosensor substrate, while an AFB1 aptamer was used as the recognition element. An AFB1-responsive aptamer-cross-linked hydrogel sensor was constructed using...
Highly sensitive detection of MUC1 by microchip electrophoresis combining with target recycling amplification and strand displacement amplification [0.03%]
结合目标循环放大和探针链替代扩增的芯片电泳高灵敏检测MUC1蛋白质
Xing Geng,Jingyi Chen,Zhaohui Chu et al.
Xing Geng et al.
Mucin 1 (MUC1) is usually overexpressed in a variety of malignant tumors, and quantitative analysis of MUC1 plays an important role in the early diagnosis of cancer. In this work, a highly sensitive MUC1 assay was developed by integrating m...
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