A Fluorescent Diaminoterephthalate Probe for Monitoring Lipid Droplet Dynamics and Ferroptosis in Mammalian Cells [0.03%]
用于监测哺乳动物细胞脂滴动力学和铁死亡的荧光二氨基均苯四甲酸探针
Shrishti P Pandey,Arkaprava Chowdhury,Swagata Bhowmick et al.
Shrishti P Pandey et al.
Ferroptosis is a Fe2+ induced programmed cell death pathway that has attracted significant attention over the last decade. It is manifested in a marked increase in the polarity of lipid droplets (LDs), as it involves lipid peroxidation. LD ...
Mild hypothermia: Insights and implications for productivity in mammalian and insect cell cultures [0.03%]
轻度低温：对哺乳动物和昆虫细胞培养生产力的影响及启示
Dayana Morales Borrell,Ziomara P Gerdtzen
Dayana Morales Borrell
Mammalian cells are the preferred expression system for obtaining recombinant proteins (r-proteins) due to their ability to generate human-like glycosylation patterns. However, their slow growth and lower productivity compared to prokaryoti...
Characterization of intermolecular base pairing using AMT crosslinking in mammalian cells during oxidative stress [0.03%]
氧化应激下AMT交联技术在哺乳动物细胞中对碱基配对的鉴定
Jeffrey N Liao,Isabel Betances,Siran Tian et al.
Jeffrey N Liao et al.
Cellular stress induces global translational repression, leading to exposure of RNA sequences that could engage in intermolecular base pairing. However, characterization of these interactions in stressed cells remains limited. Here, we coup...
Profiling Cytosolic Drug Delivery in Mammalian Cells: A Generalizable Assay for Intracellular Accumulation [0.03%]
哺乳动物细胞胞质药物递送的剖析：一种细胞内蓄积的普适性测定方法
Sobika Bhandari,George M Ongwae,Rachita Dash et al.
Sobika Bhandari et al.
The ability of biologically active molecules to access intracellular targets remains a critical barrier in drug development. While assays for measuring cellular uptake exist, they often fail to distinguish between membrane-associated or end...
Principles and functional consequences of plasmid chromatinization in mammalian cells [0.03%]
哺乳动物细胞中质粒染色质化的基本原理及其功能后果
Benjamin J Mallory,Thomas W Tullius,Carina G Biar et al.
Benjamin J Mallory et al.
Plasmids have fundamentally transformed how we resolve regulatory grammar across the tree of life. However, although chromatin plays an integral role in regulating the function of regulatory elements along the nuclear genome, our understand...
Engineered transcription factor-binding arrays for DNA-based gene expression control in mammalian cells [0.03%]
用于哺乳动物细胞基于DNA的基因表达控制的工程转录因子结合阵列
Annalise Zouein,Brittany Lende-Dorn,Kate E Galloway et al.
Annalise Zouein et al.
Tools that manipulate gene expression in mammalian cells without any additional expression are critical for cell engineering applications. Here, we demonstrate the use of arrays of transcription factor (TF) recognition elements (REs) as DNA...
Engineered Cas12j-8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells [0.03%]
工程化的Cas12j-8在哺乳动物细胞中实现多重基因组调控的平台
Ru Meng,Jiayao Li,Wuke Wang et al.
Ru Meng et al.
Cas12j-8 is a compact Cas nuclease discovered from the metagenome of giant bacteriophages, consisting of only 717 amino acids and recognizing the '5-TTN-3' protospacer adjacent motif (PAM) sequence. However, its low gene editing efficiency ...
Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells [0.03%]
一种量化哺乳动物细胞免疫检查点蛋白特定位点糖基硫酸化的方法
Wen-Cheng Chou,Wei-Ting Chen,Yuh-Shan Jou et al.
Wen-Cheng Chou et al.
Carbohydrate sulfotransferase 8 (CHST8) promotes immune checkpoint activation by sulfating GalNAcβ1-4GlcNAc (LacdiNAc) termini on glycoproteins. We present a protocol for quantifying LacdiNAc levels in mammalian cells using a lectin-captur...
Cell-cell communication-mediated cell-type-specific parent-of-origin effects in mammals [0.03%]
哺乳动物中细胞间通讯介导的细胞类型特异性印迹效应
Jia-Jin Wu,Enqin Zheng,Langqing Liu et al.
Jia-Jin Wu et al.
Genomic imprinting is manifested as monoallelic expression of genes according to parental origin, which is closely linked to mammalian placentation and human diseases. Yet, it is unclear how genomic imprinting evolves in different cell type...
Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells [0.03%]
用于识别果蝇神经干细胞和培养哺乳动物细胞有丝分裂记忆蛋白基因组结合位点的协议
Yuying Shen,Jie Liu,Yan Song
Yuying Shen
Mitotic bookmarkers label key gene loci on highly condensed chromosomes to preserve cell fate memory across mitosis. Here, we present a protocol for identifying binding sites of mitotic bookmarkers (BISMIBs) in developing tissues and cultur...
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