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Ultrasensitive and specific detection of Coxsackievirus A10 using the reverse transcription multiple cross displacement amplification combined with CRISPR/Cas12a system

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The escalating global burden of Coxsackievirus A10 (CVA10)-associated hand, foot, and mouth disease (HFMD), coupled with the lack of specific antiviral therapies, has created an urgent need for diagnostic methods that balance sensitivity, speed, and field-depl... ...