Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay
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Bovine coronavirus (BCoV) is commonly used to evaluate the efficiency of nucleic acid extraction protocols used in wastewater-based surveillance (WBS). Here we outline a method for measuring the efficiency of a solids-based extraction protocol using RT-qPCR de... ...
