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LC-MS/MS outperforms ELISA and UPLC-PDA for targeted quantification of 25(OH)D2 and 25(OH)D3 in human milk: A practical analytical strategy

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Accurate analysis of vitamin D in human milk is essential for infant nutrition but remains analytically challenging due to low levels and a complex matrix. This study evaluates a practical multi-method strategy using LC-MS/MS, ELISA, and UPLC-PDA for vitamin D... ...