In vitro CRISPR/Cas9-RNP cleavage of CcYUC1 in Coffea canephora
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An in vitro CRISPR/Cas9-RNP system efficiently cleaves CcYUC1 in Coffea canephora, establishing a foundation for DNA-free genome editing in coffee. Somatic embryogenesis depends on auxin biosynthesis and signaling; however, functional validation of candidate g... ...
