Development of species-specific SYBR green-based real-time PCR assay to differentiate between Lupinus angustifolius and Lupinus albus
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This study aimed to develop a real-time PCR assay to specifically distinguish between L. angustifolius and L. albus. Species-specific primers were designed based on sequence variations in chloroplast DNA, including the rps16-accD, matK, rbcL, and accD regions,... ...