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Sequential- vs. density gradient-centrifugation for the isolation of mitochondria-containing extracellular vesicles

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A subset of extracellular vehicles (EVs) with particle diameters >200 nm, large vesicles (lEVs), contain mitochondria that increase recipient cell bioenergetics. To date, sequential centrifugation (SC) is the most reported protocol to separate lEVs from the sm... ...