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Challenges in ITS-based identification of Cunninghamella bertholletiae and Rhizopus microsporus due to intragenomic ITS sequence heterogeneity

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Cunninghamella bertholletiae and Rhizopus microsporus are clinically important Mucorales that frequently exhibit mixed peaks in chromatograms from conventional Sanger sequencing of the internal transcribed spacer (ITS) region. Intragenomic ITS heterogeneity ha... ...