Fast Generation of F(Ab')2 Fragments From Human IgG Using Fc-Fused IgG-Degrading Enzyme
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Removal of Fc-fragments from human IgG or Fc-fused proteins. (1) IgG or Fc-fusion proteins are incubated with IdeS-Fc; (2) Fc fragments are retained using protein G agarose beads; and (3) the F(ab')2 fragments or Fc-free proteins are collected by centrifugati... ...
