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Heterologous Expression, Purification, and Biophysical Characterisation of the Cobalt-Dependent Nitrile Hydratase from Rhodococcus rhodochrous ATCC BAA-870

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Nitrile hydratases (NHases) are cobalt-dependent metalloenzymes that catalyse the hydration of nitriles to amides and are widely employed as industrial biocatalysts in the sustainable production of pharmaceuticals, polymers, and fine chemicals. Despite their b... ...