Soluble Expression, Two-Step Purification and Tagmentation-Compatible Activity Assessment of Hyperactive Tn5 Transposase Using a GB1 Fusion Tag Strategy
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Background: Hyperactive Tn5 transposase is an essential reagent for tagmentation-based next-generation sequencing (NGS) library construction that includes assays for transposase-accessible chromatin using sequencing and cleavage ... ...
