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Genome-based re-evaluation of the ECA75F sequence and development of a dual-probe real-time PCR assay for differentiating Shigella species and enteroinvasive Escherichia coli

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Shigella species and enteroinvasive Escherichia coli (EIEC) have highly similar biochemical, serological, and pathogenic profiles, making their differentiation challenging. Although the 25-base sequence ECA75F within the 16S rRNA gene has been used as a potent... ...